624 



ORDEK IV. EUBACTERIALES 



pH of glucose broth is 7.6 to 8.0 in 10 days. 



Citrates not utilized. 



Nitrites not produced from nitrates. No 

 gas produced in nitrate broth under ana- 

 erobic conditions. 



Urease not produced. 



Aerobic. No growth in glucose broth under 

 anaerobic conditions. 



Temperature relations: Optimum, be- 

 tween 35° and 40° C. Maximum, 52° C. 



Source: Isolated from an infant's stool 

 (Batchelor), from macerated figs (Saghafi 

 and Appleman) and from the surface of an 

 orange (Smith and Gordon, unpublished 

 data). 



Habitat: Uncertain. Probably associated 

 with decomposing materials. 



10. Bacillus firmus Werner, 1933. (Cent, 

 f. Bakt., II Abt., 87, 1933, 470.) 



fir'mus. L. adj. firmus strong, firm. 



Rods, 0.6 to 0.9 by 1.5 to 4.0 microns, with 

 ends poorly rounded, occurring singly or in 

 pairs. Stain uniformly. Motile. Gram-posi- 

 tive. 



Spores, 0.7 to 0.9 by 1.0 to 1.4 microns, 

 ellipsoidal, central to subterminal. Usually 

 form in 48 hours and more numerous on 

 agar without the beef extract. 



Sporangia only slightly swollen if at all. 



Gelatin stab: Slow crateriform liquefac- 

 tion. 



Gelatin agar streak plate: Wide zone of 

 hydrolysis. 



Agar colonies: Small, round, smooth, 

 dense, whitish, rarely pink. 



Agar slants: Growth moderate, flat, 

 smooth, opaque, whitish, rarely pink. 



Glucose agar slants: Growth very scant, 

 inhibited b}^ the acid produced from the 

 glucose. 



Glucose nitrate agar slants: No growth. 



Soybean agar slants: Scant, if any, 

 growth. 



Broth: Turbidity light, uniform or floc- 

 culent. 



NaCl broth: Usually there is growth in 

 5 per cent NaCl; no growth in 10 per cent. 



Milk agar streak plate: Moderate to wide 

 zone of hydrolysis of the casein. 



Potato: No growth. 



Indole not produced. 



Slight acid but no gas (with peptone as 

 source of nitrogen) from glucose. 



Starch hydrolyzed. 



Ammonium salts not utilized as source of 

 nitrogen. 



Acetylmethylcarbinol not produced. 



pH of glucose broth is not less than 6.2 in 

 7 days; very little growth. 



Citrates not utilized. 



Nitrites produced from nitrates. No gas 

 produced in nitrate broth under anaerobic 

 conditions. 



Urease not produced. 



Accessory growth substances essential. 



Lecithinase not produced. 



Aerobic. No growth in glucose broth under 

 anaerobic conditions. 



Temperature relations: Optimum, be- 

 tween 28° and 33° C. No growth at 50° C. 



Source: Isolated from soil. 



Habitat: Soil and decomposing materials; 

 also found as a laboratory contaminant. 



11. Bacillus lentus Gibson, 1935. (Zent. 

 f. Bakt., II Abt., 92, 1935, 368.) 



len'tus. L. adj. lentus slow. 



Rods, 0.6 to 0.9 by 1.5 to 4.0 microns, with 

 ends poorly rounded, occurring singly or in 

 pairs. Stain uniformly. Motile. Gram-posi- 

 tive. 



Spores, 0.7 to 0.9 by 1.0 to 1.4 microns, 

 ellipsoidal, central to subterminal. Usually 

 form in 48 hours and are more numerous on 

 agar without the beef extract. 



Sporangia only slightly swollen if at all. 



Gelatin stab: No liquefaction. 



Gelatin agar streak plate: No zone of 

 hydrolysis. 



Agar colonies: Small, round, smooth, 

 dense, whitish, not distinctive. 



Agar slants: Growth slow, thin, trans- 

 lucent becoming opaque, whitish. 



Glucose agar slants: Growth scant, not 

 so good as on agar because of the acid pro- 

 duced from the glucose. 



Glucose nitrate agar slants: No growth. 



Soybean agar slants: Very scant, if any, 

 growth. 



Broth: Faint, uniform turbidity; granular 

 sediment. 



NaCl broth: Growth in 4 per cent, none 

 in 5 per cent NaCl. 



