FAMILY XIII. BACILLACEAE 



625 



Milk: Unchanged. 



Milk agar streak plate: Casein not hj-- 

 drolyzed. 



Potato: No growth. 



Indole not produced. 



Acid but no gas (with peptone as source 

 of nitrogen) from arabinose, xylose and 

 glucose. Ammonium salts not utilized as 

 source of nitrogen. 



Starch is hydrolyzed. 



Acetj'lmethylcarbinol not produced. 



pH of glucose broth cultures is not less 

 than 6.2. 



Citrates not utilized. 



Nitrites not produced from nitrates. No 

 gas produced in nitrate broth under anaer- 

 obic conditions. 



Accessory growth factors essential. 



Lecithinase reaction doubtful. 



Urease produced. 



Aerobic. No growth in glucose broth under 

 anaerobic conditions. 



Temperature relations: Optimum, be- 

 tween 28° and 33° C. No growth at 45° C. 



Source: Isolated from soil. 



Habitat: Probably common in soil. 



12. Bacillus polymyxa (Prazmowski, 

 1880) ^Migula, 1900. {Clostridium polymyxa 

 Prazmowski, Inaug. Diss., Leipzig, 1880, 

 37; Migula, Syst. d. Bakt., 2, 1900, 638.) 



po.ly.my'xa. Or. pref. poly- much, many; 

 Gr. noun myxa slime or mucus; M.L. poly- 

 myxa much slime. 



Rods, 0.6 to 1.0 by 2.0 to 7.0 microns, not 

 in chains. Motile. Gram-variable. 



Spores, 1.2 to 1.5 by 1.5 to 2.5 microns, 

 ellipsoidal, central to sub-terminal. Spore 

 wall usually thick and easily stained. Freely 

 formed. 



Sporangia definitely bulged, spindle- 

 shaped or clavate. 



Gelatin stab: Slow liquefaction. 



Gelatin agar streak plate: Usually there 

 is a wide zone of hj^drolysis (in the case of 

 widely spreading cultures, the zone of hy- 

 drolysis sometimes extends only slightly 

 beyond the limits of growth). 



Agar colonies: Usually thin, translucent, 

 spreading, lobate or fimbriate. Rough stage 

 small, round, whitish, sometimes adherent. 



Agar slants: Growth scant to moderate. 



restricted or spreading, indistinct to whit- 

 ish. 



Glucose agar slants: Growth usually much 

 thicker than on agar, raised, glistening, 

 gummy, with production of gas. Variation: 

 Growth thin and not gummy. 



Glucose nitrate agar slants: Usually good 

 growth, gummy. 



Proteose-peptone acid agar slants: Good 

 growth, usually no gas. 



Soybean agar slants: Good growth, usu- 

 ally with the production of gas. 



Stock culture agar slants: Slightly' heavier 

 grow^th than on agar. No gas. 



Broth: Turbidity uniform to granular. 

 Gummy sediment. Sometimes a pellicle is 

 formed. 



NaCl broth: No growth in 5 per cent 

 NaCl. 



Milk: Usually coagulated with the pro- 

 duction of gas. 



Milk agar streak plate: Casein hydro- 

 lyzed. 



Potato: Growth moderate to abundant, 

 slimy, whitish to light tan. Potato decom- 

 posed with the production of gas. Growth 

 of rough strains thicker and heaped up. 



Indole not produced. 



Acid and usually gas and gum (with am- 

 monium salts as source of nitrogen) from 

 arabinose, xylose, rhamnose, glucose, lac- 

 tose, mannitol and sorbitol (the production 

 of gas can best be demonstrated by growing 

 the cultures on one of the following media: 

 agar plus 1 per cent potato starch, potato 

 plugs or wheat mash as used for the produc- 

 tion of crystalline dextrins). 



Starch hydrolyzed. Crystalline dextrins 

 not produced from starch. 



Hemicellulose and pectin attacked (An- 

 kersmit, Cent. f. Bakt., I Abt., Orig., J^0, 

 1905, 100). 



Acetylmethylcarbinol is produced. Eth- 

 anol, butylene-glycol and small amounts 

 of acetone and butanol are also produced. 



pH of glucose broth cultures is 4.8 to 7.2. 



Citrates usually not utilized. 



Methylene blue reduced; not reoxidized 

 in 21 daj'S. 



Nitrites produced from nitrates. No gas 

 produced in nitrate broth under anaerobic 

 conditions. 



