FAMILY XIII. BACILLACEAE 



629 



Milk agar streak plate: Usually scant, 

 if any, hydrolysis of the casein. 



Potato: Usually no visible growth. Vari- 

 ations : Growth scant to abundant, yellowish 

 to brownish. GummJ^ 



Indole not produced. 



Acid but no gas (with ammonium salts 

 as source of nitrogen) from glucose; acid 

 usually produced from arabinose, xylose 

 and sucrose. 



Starch is hydrolyzed. Crystalline dextrins 

 produced from starch by some strains. 



Acetylmethylcarbinol not produced. 



pH of glucose broth cultures is usually 

 less than 5.5. 



Citrates usually not utilized. 



Methylene blue usuall}^ reduced; reox- 

 idized in few days. 



Nitrites may or may not be produced 

 from nitrates. Usually no gas produced in 

 nitrate broth under anaerobic conditions. 



Urease usually not produced. 



Thiamine and biotin are essential for 

 growth. 



Lecithinase not produced. 



Aerobic, usually facultatively anaerobic. 

 Usually there is growth in glucose broth 

 under anaerobic conditions, pH 4.8 to 6.0. 



Temperature relations: Optimum, about 

 30° C. Maximum, usually between 40° and 

 45° C; with some strains, between 50° and 

 55° C. 



Source: Isolated from tap water. 



Habitat: Widely distributed in soil, water 

 and dust; also found as a laboratory con- 

 taminant. 



17. Bacillus laterosporus Laubach, 

 1916. (Jour. Bact., 1, 1916, 511.) 



la.te.ros'po.rus. L. noun latus, lateris 

 the side or flank; Gr. noun sjpora seed;M.L. 

 noun spora a spore; M.L. adj. laterosporus 

 with lateral spore. 



Rods, 0.5 to 0.8 by 2.0 to 5.0 microns, 

 sometimes slightly smaller or larger in 

 width, with ends poorly rounded or pointed. 

 Motile. Gram- variable. 



Spores, 1.0 to 1.3 by 1.2 to 1.5 microns, 

 ellipsoidal, central, formed at one side of 

 the rod. Spore wall thick and easily stained. 

 Remnants of sporangium adhering to ma- 



ture spore thicker on one side of the spore 

 than on the other. 



Sporangia definitely bulged and spindle- 

 shaped. 



Gelatin stab: Slow liquefaction. - 



Gelq,tin agar streak plate: Visible zone of 

 hydrolysis. 



Agar colonies: Thin, translucent, spread- 

 ing, irregular. Variations: Small, round, 

 rough, opaque. 



Agar slants: Growth thin, flat, spreading, 

 translucent. Variations: Growth abundant 

 or restricted. Opaque. 



Glucose agar slants: Growth heavier than 

 on agar, dense and wrinkled. 



Soybean agar slants: Growth variable. 

 \7 Broth: Turbidity uniform to granular. 



NaCl broth: Usually there is growth in 

 2 per cent NaCl; no growth in 5 per cent. 

 ' Milk: Usually curdled, peptonized. 



Milk agar streak plate: Usually there is 

 a wide zone of hydrolysis of the casein. 



Potato: Growth scant to abundant, 

 spreading, gray, pink or brown. 



Indole usually produced. 

 n/ Acid but no gas (with ammonium salts 

 as source of nitrogen) from glucose and 

 mannitol; acid usually produced from su- 

 qrose. No acid from arabinose or xylose. 

 J Starch not hydrolyzed. 



Acetylmethylcarbinol not produced. 



pH of glucose broth cultures is 6.0 to 7.4. 



Citrates not utilized. 

 J Nitrites produced from nitrates. Usually 

 a few bubbles are produced in nitrate broth 

 under anaerobic conditions. 



Urease not produced, 

 s/ Aerobic, facultatively anaerobic. Growth 

 in glucose broth under anaerobic condi- 

 tions, pH 4.8 to 5.8. 



^ Temperature relations: Optimum, be- 

 tween 28° and 37° C. No growth at 45° C. 



Source: Isolated from water. 



Habitat: Widely distributed in soil, water 

 and dust. 



18. Bacillus pulvifaciens Katznelson, 

 1950. (Jour. Bact., 59, 1950, 153.) 



pul.vi.fa'ci.ens. L. noun pulvus dust; 

 L. V. facio to make; M.L. part. adj. pulvi- 

 faciens dust-producing. 



Rods, 0.3 to 0.6 by 1.5 to 3.0 microns, 



