632 



ORDER IV. EUBACTERIALES 



starch (Foster et al., Jour. Bact., 59, 1950, 

 463). 



Temperature relations: Optimum, about 

 37° C. Ma.ximum, about 45° C. 



Pathogenicity: Cause of American foul- 

 brood of honey bees. 



Source: Isolated from scales of dead lar- 

 vae. 



Habitat: Diseased broods. 



21. Bacillus popilliae Dutky, 1940. 

 (Jour. Agr. Research, 61, 1940, 59.) 



po.pil'li.ae. M.L. noun Popillia generic 

 name of the Japanese beetle; M.L. gen. noun 

 popilliae of Popillia. 



Description taken from Dutky (loc. cit.). 



Rods, unstained, 0.9 by 5.2 microns. 

 Stained by crystal violet after fixing in 

 Schaudinn's solution, 0.3 by 3.5 microns. 

 Non-motile. Gram-positive. 



Spores, 0.9 by 1.8 microns, cylindrical, 

 central. No free spores observed. Spores 

 formed on artificial media (Steinkraus and 

 Tashiro, Science, 121, 1955, 873). 



Sporangia swollen and spindle-shaped 

 (contain a refractile body, about half the 

 size of the spore, at the broader pole of the 

 cell; this body reacts similarly to spores 

 with respect to stains). 



Unheated egg yolk beef infusion agar 

 slants: Small, discrete colonies. 



Aerobic, facultatively anaerobic. 



Temperature relations: Optimum, about 

 30° C. Maximum, about 36° C. 



Pathogenicity: Cause of type A milky 

 disease of Japanese beetle (Popillia japon- 

 ica Newm.). 



Source: Isolated from infected larvae. 



Habitat: Diseased larvae in soil. 



22. Bacillus lentimorbus Dutky, 1940. 

 (Jour. Agr. Research, 61, 1940, 65.) 



len.ti.mor'bus. L. adj. lentus slow; L. 

 noun morbus disease; M.L. noun lentimorbus 

 the slow disease. 



Description taken from Dutky (loc. cit.). 



Rods, unstained, 1.0 by 5.0 microns. 

 Stained by crystal violet after fixing in 

 Schaudinn's solution, 0.5 by 4.0 microns. 



Spores formed on artificial media (Stein- 

 kraus and Tashiro, Science, 121, 1955, 873). 



Grows on artificial media (Dutky, Jour. 



Bact., 54, 1947, 267; also see Steinkraus and 

 Tashiro, op. cit., 1955, 873). 



Aerobic, facultatively anaerobic. 



Temperature relations: Optimum, about 

 25° C. Maximum, about 30° C. 



Pathogenicity: Cause of type B milky 

 disease of Japanese beetle {Popillia japon- 

 ica Newm.). 



Source: Isolated from infected beetles. 



Habitat: Diseased larvae in soil. 



23. Bacillus pantothenticus Proom and 

 Knight, 1950. (Jour. Gen. Microbiol., 4, 

 1950, 539.) 



pan.to.then'ti.cus. M.L. adj. acidumpan- 

 tothenicum pantothenic acid; M.L. adj. 

 pantothenticus (probably intended to mean) 

 related to pantothenic acid. 



Rods, 0.4 to 0.7 by 1.2 to 3.5 microns, not 

 in chains. Stain evenly. Motile. Gram-posi- 

 tive. 



Spores, 0.8 to 1.0 by 0.8 to 1.3 microns, 

 round to ellipsoidal, terminal. Spore wall 

 thin and not easily stained. (Round spores 

 are smaller than the ellipsoidal.) 



Sporangia definitely swollen; drumstick- 

 to racket-shaped. 



Gelatin agar streak plate: Wide zone of 

 hydrolysis. 



Agar colonies: Small, round, translucent 

 to dense, granular to moire, smooth to 

 rough. Embedded colonies lenticular or 

 irregular and hairy. 



Agar slants: Growth moderate, thick, 

 not spreading, whitish. 



Glucose agar slants: Growth scant, less 

 than on agar. 



Proteose-peptone acid agar slants: No 

 growth. 



Soybean agar slants: No growth. 



Broth: Light to moderate uniform tur- 

 bidity. 



NaCl broth: Growth in 10 per cent NaCl; 

 marked stimulation by a 5 per cent concen- 

 tration. 



Milk agar streak plate: Wide zone of hy- 

 drolysis of the casein. 



Potato: No growth. 



Indole not produced. 



Acid but no gas (with ammonium salts as 

 source of nitrogen) from glucose and su- 

 crose. Acid production variable from arabi- 



