634 



ORDER IV. EUBACTERIALES 



Ann. de Micrographie, 1, 1889, 552; Migula, 

 Syst. d. Bakt., 2, 1900, 726.) 



pas.teur'i.i. M.L. gen.noun pasteurii of 

 Pasteur; named for Louis Pasteur, a French 

 chemist and bacteriologist. 



Description taken from Lohnis and 

 Kuntze (Cent. f. Bakt., II Abt., 20, 1908, 

 684), Gibson (Jour. Bact., 28, 1934, 295 and 

 313; also see ibid., 29, 1935, 491) and Smith, 

 Gordon and Clark (op. cit., 1952). 



Rods, 0.6 to 0.9 by 1.5 to 3.5 microns (1.0 

 to 1.5 by 4.0 to 5.0 microns, Lohnis and 

 Kuntze), with rounded ends, usually not 

 in chains. Motile. Gram-variable. 



Spores, 1.0 to 1.2 microns in diameter, 

 round, terminal to subterminal. 



Sporangia usually bulged and clavate. 



Urea gelatin stab : Slow, crateriform lique- 

 faction. 



Urea gelatin streak plate: Visible zone of 

 hydrolysis. 



Urea agar colonies: Small, round, not 

 distinctive. 



Urea agar slants: Growth thin, restricted, 

 translucent. 



Urea agar slants with pH 6.0 or less : No 

 growth. 



Urea soybean agar slants: Good growth, 

 heavier than on agar. 



Urea broth: Turbidity uniform, moderate 

 to heavy. 



NaCl urea broth: Growth in 10 per cent 

 NaCl. 



Urea milk agar streak plate: Hydrolysis 

 of the casein is variable. 



No acid from carbohydrates with agar 

 plus urea as base medium. 



Starch not hydrolyzed. 



Acetylmethylcarbinol not produced (urea 

 added) . 



Nitrites produced from nitrates in urea 

 nitrate broth. Gas production variable 

 under anaerobic conditions. 



Urease produced. 



Thiamine plus biotin or nicotinic acid are 

 essential for growth. Alkaline reaction and 

 usually ammonia or urea are also essential. 



Aerobic, facultatively anaerobic. Growth 

 in urea glucose broth under anaerobic con- 

 ditions. 



Temperature relations: Optimum, be- 

 tween 28° and 35° C. Maximum, between 

 37° and 40° C. 



Source: Isolated from decomposing urine. 



Habitat: Widely distributed in soil, ma- 

 nure and sewage. 



Genus II. Clostridium Prazmowski, 1880.* 



(Untersuchungen iiber die Entwickelungsgeschichte und Fermentwirkung einiger 

 Bacterien-Arten, Inaug. Diss., Leipzig, 1880, 23.) 



Clos.tri'di.um. Gr. noun closter a spindle; M.L. dim. noun Clostridium a small spindle. 



Rods, often swollen at sporulation, producing clostridial, plectridial, clavate or navicular 

 forms. Motile by means of peritrichous flagella; occasionally non-motile. Generally Gram- 

 positive. Many species are saccharolytic and fermentative, producing various acids (gen- 

 erally butyric and acetic), gases (CO2 , H2 and, at times, CH4) and variable amounts of 

 neutral products, i.e. alcohols and acetone. Other species are proteolytic, some being ca- 

 pable of attacking native and coagulated proteins with putrefaction or more complete pro- 

 teolj'sis. Several species are active in the fi.xation of free nitrogen. Strictly anaerobic or 

 anaerobic, aerotolerant. Catalase is lacking except in small amounts in certain aerotolerant 

 forms. A few species are obligately thermophilic. Exotoxins are sometimes produced. Com- 

 monly found in soil and in the intestinal tracts of man and other animals. 



The type species is Clostridium huiyricum Prazmowski. 



* Revised by Prof. R. S. Spray, School of Medicine, West Virginia University, Morgan- 

 town, West Virginia, May, 1942. Further revision by Prof. L. S. McClung, Department of 

 Bacteriology, Indiana University, Bloomington, Indiana, and Prof. Elizabeth McCoy, 

 Department of Bacteriology, College of Agriculture, University of Wisconsin, Madison, 

 Wisconsin, September, 1955. 



