664 



ORDER IV. EUBACTERIALES 



Not pathogenic for guinea pigs. 



Source: Isolated from oysters. 



Habitat : Found in oysters so far as known. 



37. Clostridium tale (Prevot at al., 

 1947) McClung and McCoy, comb. nov. (In- 

 flabilis talis Prevot, Digeon, Peyr6, Pan- 

 taloon and Senez, Ann. Inst. Past., 73, 1947, 

 416.) 



ta'le. L. adj. talis such, so great, so ex- 

 cellent. 



Straight rods, 0.7 to 0.8 by 3.0 to 5.0 mi- 

 crons. Spores subterminal, swelling the cells. 

 Not encapsulated. Non-motile. Gram-posi- 

 tive. 



Gelatin: Liquefaction. 



Agar deep colonies: Lenticular with ten- 

 dency to become irregular, evolving putrid 

 gas. 



Peptone broth: Slightly turbid. 



VF glucose broth: Abundantly turbid; 

 much gas; marked putrid odor. 



Milk: Coagulated in one to several days, 

 then digested. 



Indole produced in trace amounts. 



Glucose, fructose, galactose, sucrose, 

 sorbitol and glycerol fermented with the 

 production of gas. 



Nitrites produced from nitrates (in pres- 

 ence of maltose) by one strain. 



Coagulated protein: Slowly and partially 

 attacked. 



Anaerobic. 



Pathogenicity variable. One strain is 

 pathogenic for guinea pigs without pro- 

 ducing local lesions but with hepatic degen- 

 eration and pulmonary congestion. Toxin, 

 on inti'avenous injection, kills mouse in 

 several seconds. 



Agglutination: Sera are strain-specific. 



Source: Isolated from an acute appendix 

 and from canned fish. 



Habitat: From decomposing organic mat- 

 ter, so far as known. 



38. Clostridium mangenotii (PrOvot 

 and Zimmes-Chaverou, 1947) McClung and 

 McCoy, comb. nov. {Inflabilis mangenoti 

 (sic) Prevot and Zimmes-Chaverou, Ann. 

 Inst. Past., 73, 1947, 603.) 



man . ge . no'ti . i. M.L. gen. noun mangenotii 

 of Mangenot; named for Prof. Mangenot, 



director of the Institut intercolonial d'Adio- 

 podoum6. 



In liquid media, short, very thick rods, 

 1.6 to 1.8 by 3.0 to 4.0 microns, often ovoid, 

 with rounded ends, occurring most often 

 in chains. On agar the rods are longer, 6.0 

 to 8.0 microns, often occurring in chains or 

 filaments. Clostridial spores are formed. 

 Non-motile. Gram-positive. 



Gelatin: Liquefaction in 24 hours. 



Agar deep colonies : Irregular, with woolly 

 edges, sometimes arborescent; little gas. 



Glucose broth: Abundant, flocculating 

 growth depositing a viscous mass. Foul odor. 



Milk: Digested in 24 hours. 



Indole and a little skatole are produced. 



Hydrogen sulfide abundantly produced. 



Glucose and maltose are fermented. Lac- 

 tose, galactose, arabinose and starch are 

 not attacked. 



Nitrites not produced from nitrates. 



Coagulated serum, fibrin and coagulated 

 egg white are slowly attacked and become 

 transparent but are not liquefied. 



Anaerobic. 



Optimum temperature, 37° C. 



Not pathogenic for guinea pigs. 



Source: Isolated from African soil. 



Habitat: Soil. 



39. Clostridium lituseburense (Pre- 

 vot, 1948) McClung and McCoy, comb. nov. 

 {Inflabilis litus-eburense (sic) Prevot, Ann. 

 Inst. Past., 74, 1948, 167.) 



li.tus.e.bu.ren'se. L. noun litus coast; 

 L. noun ebur ivory; M.L. adj. lituseburensis 

 pertaining to the Ivory Coast. 



Straight rods, 1.0 by 4.0 to 6.0 microns, 

 with rounded ends, occurring in short 

 chains. Subterminal, clostridial spores. 

 Non-motile. Gram-positive. 



Gelatin: Complete liquefaction in 24 

 hours. 



Agar deep colonies: Woolly; gas is pro- 

 duced. 



Peptone broth: Slightly turbid; gas. 



VF glucose broth: Abundantly turbid; 

 gas; foul odor; non-coherent, slimy sedi- 

 ment. 



Milk: Coagulated with rapid digestion. 



Indole and skatole not produced. 



Hydrogen sulfide produced. 



