FAMILY XIII. BACILLACEAE 



C69 



Gelatin: No liquefaction. 



Gelatin deep colonies: Small, gray, fila- 

 mentous. 



Agar deep colonies: Irregular, gray, trans- 

 lucent, filamentous. 



Broth: Uniformly turbid. 



Litmus milk: Acid, but no further change. 



Potato: Gray, filamentous growth; sub- 

 stance not digested. 



Acid and gas from glucose and lactose. 

 Acid but no gas from sucrose and dulcitol. 

 Starch not fermented. 



Coagulated albumin: No liquefaction. 



Anaerobic. 



Growth occurs at 22° C. in gelatin. 



Source: Isolated from human feces. 



Habitat: Not determined. 



47. Clostridium sartagoformum Par- 

 tansky and Henry, 1935. (Jour. Bact., 30, 

 1935, 570.) 



sar.ta.go.for'mum. L. noun sartago, -inis 

 a frying pan; L. noun forma shape; L. adj. 

 formus warm; M.L. adj. sartagoformum 

 (probably intended to mean) shaped like a 

 frying pan. 



Slender, curved rods, 0.3 to 0.5 by 3.5 to 

 6.0 microns, with rounded ends, occurring 

 singly. Spores ovoid, terminal, swelling the 

 cells. Motile. Gram-positive. 



Gelatin: No liquefaction. 



Agar surface colonies (anaerobic) : Con- 

 vex, discrete, circular, transparent to white 

 and opaque. Surface moist and smooth. 



Agar deep colonies: Regular, lenticular, 

 smooth. 



Broth: No growth. Clear. 



Glucose broth: Turbid; gas bubbles. 



Litmus milk: Acid; slowly coagulated 

 withsome gasproduction. Clot not digested. 



Potato: Very scant growth. No gas in sur- 

 rounding liquid. 



Indole not produced. 



Acid and gas from xylose, glucose, fruc- 

 tose, galactose, sucrose, lactose, maltose, 

 raffinose, inulin, salicin, mannitol, acetate 

 and butyrate. Starch, ethanol, glycerol and 

 dulcitol not fermented. 



Nitrites not produced from nitrates. 



Coagulated albumin: No liquefaction. 



Blood agar: No hemolysis. 



Blood serum: No liquefaction. Scant 

 growth. 



Brain medium: No blackening or diges- 

 tion. Some gas is produced. 



Anaerobic. 



Optimum temperature, 37° C. 



Distinctive character: Ferments sulfite- 

 waste liquor in 40 per cent concentration, 

 producing butyric and acetic acids, H2 and 

 CO2. 



Source: Isolated from garden soil and 

 from stream and lake mud. 



Habitat: Presumably soil. 



48. Clostridium paraputrificum (Bien- 

 stock, 1906) Snyder, 1936. (Art V, Bienstock, 

 Fortschr. d. Med., 1, 1883, 612; Bacillus 

 diaphthirus Trevisan, I generi e le specie delle 

 Batteriacee, 1889, 15; Bacillus paraputrificus 

 Bienstock, Ann. Inst. Past., 20, 1906, 413; 

 also see Strassburger Med. Zeit., 3, 1906, 111 ; 

 Snyder, Jour. Bact., 32, 1936, 401.) 



pa.ra.pu.tri'fi.cum. Gr. pref. para beside, 

 by; M.L. noun putrijicum a specific epithet; 

 M.L. adj. parapw^rz^cws resembling (Clostri- 

 diuvi) putrificum. 



Description taken from Hall and Synder 

 (Jour. Bact., 28, 1934, 181). 



Straight or slightly curved rods, 0.3 to 

 0.5 by 2.0 to 6.0 microns, with rounded ends, 

 occurring singly, in pairs or in short chains. 

 Spores ovoid, terminal, swelling the cells. 

 Motile by means of peritrichous flagella. 

 Gram-positive. 



Gelatin : No liquefaction. Gas is produced. 



Agar deep colonies: Small, irregular, 

 opaque, dense, cottony masses. Gas is pro- 

 duced. 



Blood agar surface colonies (anaerobic) : 

 Delicate, irregular, round- topped dew- 

 drops. No hemolysis. 



Broth: Diffuse turbidity. 



Milk: Usually coagulated in from 6 to 10 

 days. Abundant gas, but no peptonization. 



Indole not produced. 



Acid and gas from glucose, fructose, galac- 

 tose, maltose, lactose, sucrose, raffinose, 

 dextrin, soluble starch, amygdalin and 

 salicin. Xylose, inulin, mannitol and glyc- 

 erol not fermented. 



Nitrates reduced (Reed, Jour. Bact., 44 1 

 1942,425). 



Coagulated albumin: No liquefaction. 



Blood serum: No liquefaction or dis- 

 coloration. 



