670 



ORDER IV. EUBACTERIALES 



Brain medium: No blackening or diges- 

 tion. Non-proteolytic. 



Anaerobic. 



Grows well at 37° C. 



Not pathogenic for guinea pigs or rabbits. 



Source: Isolated from feces and gaseous 

 gangrene and from post-mortem fluid and 

 tissue cultures. 



Habitat : Presumably occurs commonly in 

 the intestinal canals of human beings. 



49. Clostridium indologenes (Prevot, 

 1948) McClung and McCoy, comb. nov. 

 (Pleciridium indologenes Prevot, Ann. Inst. 

 Past., 74, 1948, 165.) 



in.do.lo'ge.nes. M.L. neut.n. indolum 

 indole; Gr. v. gennaio to produce; M.L. adj. 

 indologenes indole-producing. 



Straight rods, 0.6 by 3.0 to 4.0 microns, 

 occurring singly or in pairs. Spores rare, 

 ovoid, large-sized and distinctly terminal. 

 Motile in very young cultures. Gram-posi- 

 tive. 



Gelatin: No liquefaction. 



Agar deep colonies: Lenticular with sec- 

 ondary off -shoots; little gas produced, if 

 any. 



Peptone broth: Turbid. 



Glucose broth: Abundantly turbid; vis- 

 cous sediment; putrid odor. 



Milk: No change. 



Indole produced. 



Hydrogen sulfide produced. 



Glucose, lactose and galactose are fer- 

 mented . 



Nitrites not produced from nitrates. 



Coagulated proteins: Not attacked. 



Anaerobic. 



Optimum temperature, 37° C. 



Optimum pH, 7.8. 



Not pathogenic for guinea pigs. 



Source: Isolated from a sample of "poto- 

 poto" in Africa. 



Habitat: Not determined. 



50. Clostridium cochlearium (Bulloch 

 et al., 1919) Bergey et al., 1923. {Bacillus 

 Type IIIc, Mcintosh, Med. Res. Counc, 

 Spec. Rept. Ser. No. 12, 1917, 20; Bacillus 

 cochlearius Bulloch, Bullock, Douglas, 

 Henry, Mcintosh, O'Brien, Robertson and 

 Wolf, Med. Res. Counc, Spec. Rept. Ser. 



No. 39, 1919, 40; Bergey et al., Manual, 1st 

 ed., 1923, 333.) 



coch.le.a'ri.um. L. noun coclear (cochlear) 

 a spoon; M.L. adj. cochlearius spoon-like. 



Straight, slender rods, occurring chiefly 

 singly and infrequently in pairs or short 

 chains. Spores ovoid, terminal, swelling the 

 cells. Motile by means of peritrichous 

 flagella. Weakly Gram-positive. 



Gelatin: No liquefaction. 



Agar surface colonies (anaerobic) : Circu- 

 lar, clear, entire or crenated. 



Agar deep colonies: Lenticular, entire. 



Broth: Turbid. 



Litmus milk: Unchanged. 



Glucose and other carbohydrates not 

 fermented. 



Coagulated albumin: No liquefaction. 



Blood serum: No liquefaction. 



Brain medium: No blackening or diges- 

 tion. 



Meat medium: Slightly reddened. No 

 blackening or digestion. Little gas produced 

 of non-putrefactive odor. 



Anaerobic. 



Optimum temperature, between 30° and 

 35° C. 



Not pathogenic. 



Source : Isolated from human war wounds 

 and septic infections. 



Habitat: Not determined. Probably oc- 

 curs in soil. 



51. Clostridium kluyveri Barker and 

 Taha, 1942. {Clostridium kluyverii (sic) 

 Barker and Taha, Jour. Bact., 43, 1942, 

 347.) 



kluy've.ri. M.L. gen. noun kluyveri of 

 Kluyver; named for Prof. A. J. Kluyver of 

 Delft, Holland, in whose laboratory this 

 organism was discovered. 



Straight to slightly curved rods, 0.9 to 

 1.1 by 3.0 to 11.0 microns, usually occurring 

 singly and in pairs, occasionally in long 

 chains. Spores ovoid, 1.3 by 1.8 microns, 

 terminal, swelling the cells. Motile by means 

 of peritrichous flagella. Generally Gram- 

 negative; some strains are weakly Gram- 

 positive when young. 



Iron-gelatin (Spray) : No growth. 



Agar surface colonies (anaerobic) : Growth 

 slow and restricted by residual traces of 



