FAMILY XIII. BACILLACEAE 



673 



Optimum temperature, 37° C. Grows well 

 at room temperature. 



Neither toxin nor hemolysin produced. 



Not pathogenic for guinea pigs or rabbits. 



Source: Isolated from a human cadaver 

 and from the peritoneum of a rabbit. 



Habitat: Not determined. 



55. Clostridium saprogenes (Salus, 

 1904) McClung and McCoy, comb. nov. 

 (Bacillus saprogenes carnis Salus, Arch. f. 

 Hyg., 51, 1904, 114; Bacillus saprogenes 

 Salus, ibid., 115; Plectridium saprogenes 

 Prevot, Ann. Inst. Past., 61, 1938, 87; also 

 see Prevot and Weislitz, Ann. Inst. Past., 

 72, 1946, 444.) 



sap.ro'ge.nes. Gr. adj. saprus rotten; Gr. 

 V. gennaio to produce; M.L. part. adj. 

 saprogenes rot-producing. 



Rods, 1.5 by 8.0 microns, curved, in chains 

 and filaments. Spores ovoid, terminal. Mo- 

 tile. Gram-positive. 



Gelatin: Slow liquefaction. 



Agar deep colonies: Lenticular or spheri- 

 cal; gas is produced. 



Liquid media: Gas production; disagree- 

 able odor. 



Glucose broth: Turbid; sediment; pene- 

 trating, putrid odor due in part to hydrogen 

 sulfide. 



Milk: Coagulated with a disagreeable 

 odor. 



Glucose, fructose, maltose, galactose, su- 

 crose, lactose, arabinose, xylose, sorbitol, 

 dulcitol, inulin and starch are fermented. 

 Glycerol and mannitol are slightly attacked. 



Nitrites not produced from nitrates. 



Coagulated egg, serum, fibrin and brain: 

 Not attacked. 



Anaerobic. 



Optimum temperature, 37° C. 



Not pathogenic. 



Source: Isolated from spoiled meat and 

 from an industrial fermentation sample. 



Habitat: Probably soil. 



56. Clostridium perenne (Prevot, 1940) 

 McClung and McCoy, comb. nov. (Acuformis 

 perennis Prevot, Compt. rend. Soc. Biol., 

 Paris, 133, 1940, 576.) 



per.en'ne. L. adj. perennis lasting through 



the year or through many years, perennial. 



Straight rods, 0.3 to 0.4 by 1.6 to 3.0 



microns for non-sporulating cells and 0.5 

 by 3.0 to 5.0 microns for sporulating cells, 

 with rounded ends, occurring in pairs or in 

 chains of 3 to 16 cells. Spores ovoid, termi- 

 nal, measuring 0.6 by 1.0 micron. Non- 

 motile. Gram-positive. 



Gelatin: No liquefaction. 



Agar deep colonies: Lenticular, whitish, 

 1 to 2 mm in diameter. Agar is split by gas. 



Glucose broth: Abundantly turbid; gas 

 liberated has a slight, but not disagreeable, 

 odor of volatile acids. 



Peptone broth: Abundantly turbid; no 

 gas. 



Milk: Rapidly acidified, then coagulated 

 with liberation of gas and retraction of clot. 



Indole not produced. 



Hydrogen sulfide not produced. 



Glucose, fructose, maltose, galactose, 

 sucrose, arabinose and lactose are strongly 

 fermented. 



Nitrites not produced from nitrates. 



Anaerobic. 



Optimum temperature, between 33° and 

 37° C. 



Optimum pH, about 7.0. 



Not pathogenic for guinea pigs, mice or 

 rabbits. 



Source: Isolated from a case of chronic 

 appendicitis. 



Habitat: Not determined. 



57. Clostridium thermosaccharolyti- 



cum McClung, 1935. (Jour. Bact., 29, 

 1935, 200.) 



ther.mo.sac.cha.ro.ly'ti.cum. Gr. adj. 

 thermus hot; Gr. noun saccharum sugar; Gr. 

 adj. lyticus dissolving; M.L. adj. thermo- 

 saccharolyticus (presumably intended to 

 mean) thermophilic and sugar-fermenting. 



Slender, granulated rods, 0.4 to 0.7 by 3.5 

 to 7.5 microns, occurring singly and in pairs, 

 not in chains. Spores spherical, terminal, 

 swelling the cells. Motile by means of peri- 

 trichous flagella. Gram-negative. 



Gelatin: No liquefaction. 



Pea-infusion agar surface colonies (anae- 

 robic) : Granular, grayish white, raised 

 center, feathery edges. 



Glucose-tryptone agar deep colonies: 

 Small, lenticular, smooth. 



Liver-infusion broth over liver meat: 

 Turbid; gas produced. 



