674 



ORDER IV. EUBACTERIALES 



Litmus milk: Litmus reduced. Acid and 

 slow but firm coagulation; coagulum split 

 with gas. Clot not digested. 



Indole not produced. 



Acid and gas from arabinose, fructose, 

 galactose, glucose, mannose, xylose, cello- 

 biose, lactose, maltose, sucrose, trehalose, 

 dextrin, glycogen, maize starch, amygdalin, 

 esculin, alpha-methyl-glucoside and salicin. 

 Raffinose weakly fermented. Rhamnose, 

 inulin, pectin, erythritol, inositol, mannitol, 

 glycerol, quercitol and Ca-lactate not fer- 

 mented. Sorbitol and dulcitol not fermented 

 (Mercer and Vaughn, Jour. Bact., 6£, 1951, 

 27). 



Cellulose not fermented. 



Nitrites not produced from nitrates. 



Coagulated albumin: No liquefaction. 



Blood serum: No liquefaction. 



Brain medium: No blackening or diges- 

 tion. 



Meat medium: No blackening or diges- 

 tion. 



Anaerobic. 



Optimum temperature, between 55° and 

 62° C. Thermophilic. 



Not pathogenic on feeding to white rats 

 or on injection to rabbits. 



Distinctive character: Differentiated 

 from Clostridium tariarivorum Mercer and 

 Vaughn by not fermenting tartrate. 



Source: Isolated from hard-swell of 

 canned goods and from soil. 



Habitat: Not determined. 



58. Clostridium indolis McClung and 

 McCoy, nom. nov* {Terminosporus indolo- 

 genes Bezjak, Ann. Inst. Past., 82, 1952, 

 101.) 



in'do.lis. M.L. neut.n. indolum indole; 

 M.L. gen. noun indolis of indole. 



Straight to slightly curved rods, 0.6 by 

 3.0 to 4.0 microns. Spores terminal, round, 

 measuring 1.0 to 1.5 microns. Motile. Gram- 

 negative. 



Gelatin: No growth. 



Veillon agar colonies: Small, lenticular; 

 no gas. 



Blood agar colonies : After 3 days incuba- 



tion, 2 mm in diameter, moist, grayish, 

 round, irregular; there is also a spreading 

 tendency. 



Glucose broth: Uniformly turbid. 



Milk: Acid and coagulated in 2 days; gas. 



Indole is produced. 



Hydrogen sulfide is produced. 



Acid and gas from glucose, sucrose, 

 maltose and lactose. Mannitol not fer- 

 mented. 



Coagulated proteins: Not attacked. 



Anaerobic. 



Grows well at 37° C. but not at ordinary 

 temperatures nor at 46° C. 



Pathogenicity: Mice injected peritoneally 

 show no efi'ects. 



Source: Isolated from a patient operated 

 on for cancer of the large intestine. 



Habitat: Probably the human intestine. 



59. Clostridium caloritolerans Meyer 

 and Lang, 1926. (Jour. Inf. Dis., 39, 1926, 

 321.) 



ca.lo.ri.to'le.rans. L. noun calor heat; 

 L. part. adj. tolerans tolerating; M.L. 

 caloritolerans heat-tolerating. 



Rods, 0.5 to 0.8 by 8.0 to 10.0 microns, 

 with rounded ends, occurring singly, in 

 pairs, in chains and in curved filaments. 

 Spores spherical or pear-shaped, terminal, 

 swelling the cells. Motile by means of peri- 

 trichous flagella. Gram-positive. 



Gelatin: No liquefaction. 



Liver agar deep colonies: Small, flat, 

 transparent discs with large, polar tufts. 

 Some colonies become fluffy. 



Glucose blood agar surface colonies 

 (anaerobic): Small, flat, grayish, rhizoidal. 

 No hemolysis. 



Broth: Slightly turbid. 



Glucose broth: Abundantly turbid; clear- 

 ing by sedimentation; gas is produced. 



Brom cresol purple milk: No change. 



Indole not produced. 



Acid and gas from glucose, galactose and 

 maltose. Fructose feebly fermented. Lac- 

 tose, sucrose, raffinose, inulin, salicin, 

 mannitol, inositol and glycerol not fer- 

 mented. 



*The specific epithet indolis is used here in lieu of indologenes because the latter is pre- 

 occupied in the genus Clostridium for an earlier-described organism (see species number 49, 

 Clostridium indologenes McClung and McCoy). 



