FAMILY XIII. BACILLACEAE 



675 



Coagulated albumin: No liquefaction. 



Blood serum: No liquefaction. 



Brain medium: No blackening or diges- 

 tion. 



Beef-heart mash medium: Reddened; no 

 blackening or digestion. 



Anaerobic. 



Optimum temperature not determined. 

 Grows at 37° C. 



Not pathogenic for mice, guinea pigs or 

 rabbits. 



Source: Isolated from an old culture of 

 Clostridium parabotulinujn Type A. 



Habitat: Not determined. 



60. Clostridium tetanoides (Adamson, 

 1918) Hauduroy et al., 1937. (Unnamed ana- 

 erobe, Adamson and Cutler, Lancet, /, 

 1917, 688; Bacillus tetanoides (A) Adamson, 

 Jour. Path, and Bact., £2, 1918-19, 382; 

 Hauduroy et al., Diet. d. Bact. Path., 1937, 

 140.) 



te.ta.no.i'des. M.L. noun tetani a specific 

 epithet; Gr. noun eidus shape, form; M.L. 

 adj. tetanoides {Clostridium) tetani-\i\iQ. 



Rods, 1.0 to 2.0 by 4.0 to 12.0 microns 

 (averaging 1.0 to 1.5 by 6.0 to 7.0 microns), 

 with rounded to slightly tapered ends, 

 occurring singly, in pairs and in chains of 3 

 to 5 cells but not in filaments. Spores large, 

 spherical, terminal, swelling the cells. 

 Motile only in young cultures. Gram- 

 positive in young cultures, soon becoming 

 Gram-negative. 



Gelatin: No liquefaction. 



Plain agar surface colonies (anaerobic) : 

 Confluent, becoming an opaque film. Iso- 

 lated colonies are circular to slightly irregu- 

 lar. Dendritic branching and mucoid 

 tendency less evident than on glucose agar. 



Glucose agar surface colonies (anaerobic) : 

 Circular, regular, opaque, bluish gray, 

 moist, shining, thick, raised. Surface flat, 

 becoming conical in center with age. On 

 moist medium, shows radiating dendritic 

 branching. Growth becomes tenacious- 

 mucoid. 



Glucose agar stab: Thick growth along 

 stab, starting 0.5 cm below surface. No gas 

 or splitting of medium. 



Neutral-red glucose agar: Reduced to 

 orange by transmitted, and to greenish 

 fluorescent by reflected light. 



Plain broth: Early, slight turbidity; 

 clearing and mucoid sedimentation. 



Glucose broth: Abundantly turbid; pro- 

 fuse, mucoid sediment. 



Milk: Slight and slowly increasing alka- 

 linity; slow separation of casein. No further 

 change. 



Indole produced in trace amounts in 

 broth. 



Acid but no gas from glucose and maltose. 

 Lactose, sucrose, mannitol, starch and 

 cellulose not fermented. 



Coagulated albumin: No digestion or 

 blackening. 



Blood serum: No digestion or blackening. 



Meat medium: No digestion or blacken- 

 ing. 



Brain medium: No digestion or blacken- 

 ing. 



Anaerobic. 



Optimum temperature not recorded. 

 Grows well at 37° C. 



Not pathogenic for guinea pigs or rabbits. 



Source: Isolated from war wounds, from 

 postmortem blood cultures and from garden 

 soil. 



Habitat: Not determined. 



61. Clostridium tartarivorum Mercer 

 and Vaughn, 1951. (Jour. Bact., 62, 1951, 

 36.) 



tar.ta.ri'vo.rum. M.L. noun acidum 

 tartaricum tartaric acid; L. v. voro to de- 

 vour; M.L. adj. tartarivorus tartrate- 

 destroying. 



Long, uniformly slender, granulated rods; 

 older cells are slightly curved. Cells, usu- 

 ally measuring 0.4 to 0.6 by 5.0 to 6.8 mi- 

 crons, vary in size depending on the growth 

 medium. Occur singly or in pairs. Spores 

 large (0.7 to 2.0 microns), ellipsoidal to 

 spherical and generally terminal. Sluggishly 

 motile by means of peritrichous flagella. 

 Gram-negative. 



Gelatin: No liquefaction. 



Green pea agar surface colonies: Large 

 (10.5 to 12.0 mm in diameter), grayish cream 

 in color. Colonies on tartrate agar are 

 smaller. 



Indole not produced. 



Hydrogen sulfide not produced from 

 tryptone (peptone); production variable 

 from sodium thiosulfate. 



