FAMILY XIII. BACILLACEAE 



685 



Biol., Paris, 99, 1928, 1517; Plectridio ama- 

 rillo, Soriano, Tomo commemorativo del 

 XXV aniversario de la fundacion de la Fa- 

 cultad de Agronomia y Veterinaria, Buenos 

 Aires, 1929 (?) ; Bacillus haumani (sic) Sori- 

 ano, Rev. Inst. Bact., Buenos Aires, 5, 1930, 

 743; Plectridium amarillum Stampa, Ann. 

 Brass, et Distill., 29, 1930-31, (253, 271 and 

 302?); Clostridium haumanni (sic) Prdvot, 

 Ann. Inst. Past., 61, 1938, 81; Clostridium 

 felsineum var. haumanni (sic) Heyn, Ver- 

 hand. d. k. Akad. v. Wetensch., Anasterdam, 

 Tweedie Reeks, 48, 1951, 29.) 



hau.man'i.i. M.L. gen. noun haumanii of 

 Hauman; named for Prof. Lucien Hauman, 

 Microbiologist of the University of Buenos 

 Aires. 



Rods, averaging 0.7 to 0.8 by 4.0 to 10.0 

 microns, occurring singly or in short chains. 

 Terminal, ovoid spores, 1.6 by 3.0 microns, 

 with spore cap. Actively motile. Granulose- 

 negative. Gram-positive, becoming Gram- 

 negative. 



Gelatin: No liquefaction. 



Carrot agar deep colonies : Lenticular with 

 smooth edges. Gas production disrupts agar. 

 Intense canary-yellow pigment diffuses in 

 medium. 



Carrot juice: Uniformly turbid. 



Potato mash : Produces good growth with 

 slow gas and with yellow pigment produc- 

 tion. No head formation as with Clostridium 

 jelsinexim Bergey et al. 



Acid and gas from amygdalin, salicin, 

 mannitol, arabinose, xylose, glucose, fruc- 

 tose, mannose, galactose, sucrose, maltose, 

 lactose, pectin and rhamnose. Arabitol, 

 dulcitol, sorbitol, inositol, raffinose, inulin 

 and starch not fermented. 



Retting action: Positive in three days. 



Anaerobic. 



Optimum temperature, 37° C. 



Source: Isolated from retting liquid from 

 Argentina. 



Habitat: Found in decomposing plant 

 materials. 



79. Clostridium virens (Prevot, 1946) 

 McClung and McCoy, comh. nov. {Plec- 

 tridium virens Prevot, Ann. Inst. Past., 72, 

 1946, 665.) 



vi'rens. L. v. vireo to be green; L. part, 

 adj. virens becoming green. 



Straight rods, 0.6 to 0.8 by 3.0 to 4.0 mi- 

 crons. Terminal, spherical spores, 1 micron 

 in diameter. Gram-positive in young cul- 

 tures. 



Gelatin: No growth. 



Glucose agar deep colonies: Colonies ap- 

 pear slowly in 5 to 8 days; transparent, 

 large, woolly colonies with green, fluorescent 

 coloration. Sometimes colonies are smaller, 

 opaque, irregular-edged and green-pig- 

 mented. Pigment is soluble and diffuses 

 completely into the agar but stops at the 

 level of the aerobic zone. 



VF glucose broth: Slow growth at 26° C. 

 in 8 to 10 days; after 15 days the broth is 

 colored green; no gas. 



Milk: No growth. 



Indole not produced. 



Potato mash: Strongly colored green in 

 2 days; marked production of gas. 



Lactose and galactose are strongly fer- 

 mented. Fructose is more weakly fermented, 

 while glucose, maltose, sucrose, sorbitol, 

 mannitol and starch are not fermented. 



Cellulose is not attacked (Prevot, Man. d. 

 Class, et d. D^term. d. Bact. Anaerob., 

 2« ed., 1948, 210). 



Propionic and formic acids are produced. 



Nitrites produced from nitrates. 



Coagulated egg white: No growth. 



Liver, brain and fibrin: No growth. 



Anaerobic. 



Temperature relations: Optimum, 26° C. 

 Weak growth at 18° and no growth above 

 30° C. Inhibition of pigment production in 

 second-generation cultures at 30° C. 



Not pathogenic for experimental animals. 



Source: Isolated from pond and river 

 muds. 



Habitat: Presumably mud. 



80. Clostridium spumarum (Prevot 

 and Pochon, 1939) Spray, 1948. {Plectridium 

 spumarum Prevot and Pochon, Compt. rend. 

 Soc. Biol., Paris, 130, 1939, 966; Spray, in 

 Manual, 6th ed., 1948, 808.) 



spu.ma'rum. L. noun spumn foam, froth; 

 L. gen.pl. noun spuviarum of foams. 



Rods, 0.5 by 4.0 microns. Spores are ovoid 

 and terminal, swelling the cells. Motile. 

 Gram-positive. 



Gelatin: Liquefaction in 15 days. 



