FAMILY I. MYCOBACTERIACEAE 



and waxy, off-white to cream-colored 

 (Gordon and Smith). 



Glycerol potato: Poor growth. 



Broth: Thin ring pellicle; broth clear. 



Milk not coagulated. 



Milk agar plate: No hydrolysis of casein 

 (Gordon and Smith). 



Indole not produced. 



Hydrogen sulfide not produced (Penso 

 et al.). Hydrogen sulfide production vari- 

 able (Gordon and Smith, unpublished data). 



Acid from glucose, mannose, trehalose 

 and occasionally mannitol. No acid from 

 rhamnose, xylose, arabinose, galactose, sor- 

 bitol, inositol, dulcitol, lactose or raffinose. 

 Maltose hydrolyzed by only one culture 

 (Gordon and Smith). 



Starch is hydrolyzed (Gordon and Smith). 



Succinate and malate used as sole sources 

 of carbon; citrate used by all but one culture 

 (Gordon and Smith). 



Tyrosine not decomposed (Gordon and 

 Smith). 



Benzoate not utilized (Gordon and 

 Smith). 



Nitrites usuallj^ produced from nitrates. 



Temperature relations: Growth at 28° to 

 35° C.; variable at 10° C.; most cultures 

 grow at 40° C.; growth, if any, scant at 

 45° C. Does not survive 60° C. for 4 hours 

 (Gordon and Smith). 



Salt tolerance: Usually growth in broth 

 containing 5 per cent NaCl; usually none 

 in 7 per cent (Gordon and Smith). 



Pathogenicity: Guinea pigs, rabbits and 

 mice resistant to massive doses of j^oung 

 cultures (Cruz). Local lesions produced in 

 lymph glands and kidneys of mice, guinea 

 pigs, rabbits, monkeys and calves (Penso 

 et al.). 



Source : Several strains were isolated from 

 lymph glands of cattle by F. D. Minett, from 

 human abscesses by Cruz, from a systemic, 

 nodular infection of Gia by Darzins. 



Habitat: Found in soil and infections of 

 humans, cattle and cold-blooded animals. 



4. Mycobacterium niarinum Aronson, 

 1926. (Jour. Inf. Dis., 39, 1926, 315.) 



ma.ri'num. L. adj. marinus of the sea, 

 marine. 



In lesions, short, thick, uniformly staining 

 organisms are seen frequently occurring in 



clumps, while long, thin, beaded or barred 

 rods are scattered more discretely. In cul- 

 tures the organisms have the same appear- 

 ance. Non-motile. Acid-fast and acid-al- 

 cohol-fast. Gram-positive. 



Gelatin: No liquefaction. 



Agar slant (slightly acid) : In five to seven 

 days, moist, glistening, elevated colonies, 

 becoming lemon-yellow. 



Agar colonies: In 5 to 7 days, smooth, 

 moist, slimy, lemon-yellow, later orange- 

 colored. 



Glycerol agar colonies: In 14 to 18 days, 

 grayish white, moist, elevated with irregular 

 margins. Old growths lemon-yellow and still 

 later orange-colored. 



Dorset's and Petroff's egg media: Similar 

 to growth on glycerol agar but more 

 luxuriant. 



Broth and glycerol broth: Growth is dif- 

 fuse; no pellicle formed. 



Litmus milk: Acidified and coagulated. 



Indole not produced. 



Arabinose and fructose are utilized; sor- 

 bitol and galactose are not utilized (Gordon, 

 Jour. Bact., 34, 1937, 617) 



Nitrites not produced from nitrates. 



Aerobic, facultatively anaerobic. 



Optimum temperature, between 18 and 

 20° C. Fails to survive 60° C. for 1 hour; 

 fails to grow at 45° C. (Gordon, Jour. Bact., 

 34, 1937, 617). 



Pathogenicity: Experimentally infects 

 salt-water fish, goldfish, frogs, mice and 

 pigeons but not rabbits or guinea pigs. 



Source: Isolated from areas of focal ne- 

 crosis of the liver of sergeant majors (Abri- 

 defduf mauritii), croakers {Micropogon 

 undulatus) and sea bass (Centropristes 

 striatus) . 



Habitat: Causes spontaneous tuberculo- 

 sis in salt-water fish. 



5. Mycobacterium thamnopheos Aron- 

 son, 1929. (Jour. Inf. Dis., 44, 1929, 215.) 



tham.no'phe.os. Gr. noun thamnus a 

 bush; Gr. noun ophis, opheos a snake; M.L. 

 mas.n. Thamnophis bush snake, a genus of 

 snakes; M.L. gen. noun thamnopheos of the 

 bush snake. 



Original description supplemented by ma- 

 terial taken from Bynoe (Thesis, McGill 

 University, Montreal, 1931). 



