FAMILY II. ACTINOMYCETACEAE 



721 



Serum agar: Minute, round, colorless 

 colonies with pinkish tinge in confluent, 

 raised patch. 



Inspissated serum: Small, round, pale 

 pink colonies; umbilicated and raised up. 



Broth: Liberal growth, white flocculent 

 colonies; later pink surface colonies. 



Sj^nthetic sucrose solution: Colorless, 

 flocculent sediment; thin, colorless pellicle. 



Milk: Surface growth; white aerial my- 

 celium; solid coagulum; later partly pep- 

 tonized with pink aerial mj'^celium. 



Litmus milk: Pink surface growth; 

 aerial mj'celium; milky opaque after 40 

 days. 



Carrot plug: Small, irregularly round, 

 raised colonies, colorless, covered with stiff 

 aerial spikes; later buff-colored, convo- 

 luted and ribbed growth with small patches 

 of white, aerial mycelium; aerial mycelium 

 pink in two months. 



Source: Isolated from a fatal case of lung 

 disease and pericarditis in man. 



Habitat: Human infections so far as 

 known. 



7. Nocardia caprae (Silberschmidt, 1899) 

 Waksman and Henrici, 1948. (Streptothrix 

 caprae Silberschmidt, Ann. Inst. Past., 13, 

 1899, 841 ; Waksman and Henrici, in Manual, 

 6th ed., 1948, 899.) 



cap'rae. L. noun capra a she-goat; L. 

 gen. noun caprae of a she-goat. 



Description taken from Erikson (Med. 

 Res. Council Spec. Rept. Ser. 203, 1935, 26). 



Initial cells only slightly enlarged; early 

 development of aerial hyphae while sub- 

 stratum threads are still short; frequent 

 slipping of branches; aerial mycelium abun- 

 dant on all media with tendency to form co- 

 herent spikes; mycelium not very polymor- 

 phous, but occasional, thicker segments 

 appear. Slightly acid-fast. 



Gelatin: Extensive dull growth with 

 small, raised patches of pink, aerial myce- 

 lium; later ribbon-like, depressed. No lique- 

 faction. 



Glucose agar: Irregular, bright pink 

 growth tending to be heaped up; later abun- 

 dant masses frosted over with thin, white, 

 aerial mycelium. 



Glycerol agar: Abundant growth; small 



round pink colonies partly covered with 

 white aerial mycelium. 



Potato agar: Extensive, thin growth, pink 

 in raised patches, covered by white, aerial 

 mycelium; later aerial mycelium also be- 

 comes pink. 



Starch agar: Minute, colorless colonies 

 covered by white, aerial mycelium. 



Blood agar: Minute, round, colorless 

 colonies aggregated in broad, pink zones; 

 paler aerial mycelium. No hemolysis. 



Dorset's egg medium: Few, colorless 

 colonies, some pink; white aerial mycelium; 

 later, growth becoming dull pink, irregular, 

 with scant white aerial mj^celium. 



Ca-agar: Minute, colorless colonies; white 

 aerial mycelium; later a pinkish tinge. 



Serum agar: Small, round, pink colonies 

 frosted over with thin, white, aerial my- 

 celium. 



Inspissated serum: No growth. 



Broth: Superficial pellicle composed of 

 pink colonies with white aerial mycelium; 

 moderate, flocculent sediment. 



Glucose broth: Small sediment of fine 

 flocculi; later pellicle composed of small 

 pink colonies; superficial skin entire and 

 salmon-colored in 16 days. 



Synthetic glycerol solution: Round, pink, 

 disc-like colonies on surface and tenuous, 

 white, wispy growth in suspension and sedi- 

 ment; after 20 days, surface colonies bearing 

 white aerial mycelium extending 2 cm up 

 tube. 



Synthetic sucrose solution: Minute, white 

 colonies in suspension and sediment in 3 

 days; thin, dust-like pellicle in 10 days; 

 some surface colonies with white aerial 

 mycelium in 17 days. 



Milk: Red surface skin; solid coagulum. 



Litmus milk: Red surface growth; no 

 change in liquid; after 4 weeks, liquid de- 

 colorized, opaque. 



Potato plug: Abundant growth; small 

 colonies, mostly confluent, entirely covered 

 with pale pink aerial mycelium; growth be- 

 comes membranous, considerably buckled; 

 later superficial colonies with pink aerial 

 mycelium on liquid at base of tube; bottom 

 growth of round white colonies. 



Starch not hydrolyzed. 



Source: Isolated from lesions in goats. 



