•22 



ORDER V. ACTINOMYCETALES 



Habitat: Found in infections of goats so 

 far as known. 



8. Nocardia pretoriana Pijper and Pul- 

 linger, 1927. (Jour. Trop. Med. and Hyg., 

 30, 1927, 153.) 



pre.to.ri.a'na. M.L. adj. pretorianus 

 pertaining to Pretoria; named for Pretoria, 

 South Africa. 



Description taken from Erikson (Med. 

 Res. Council Spec. Rept. Ser. 203, 1935, 

 30). 



Minute, fiat colonies are formed consisting 

 of angularly branched filaments and bearing 

 a few short, straight aerial hyphae; later the 

 growth becomes spreading and extensive, 

 the slipping of the branches is well marked 

 and the aerial hyphae are divided into 

 cylindrical conidia. Slightly acid-fast. 



Gelatin: A few, colorless flakes. No lique- 

 faction. 



Glucose agar: Pale buff, umbilicated and 

 piled up colonies. 



Glycerol agar: Piled up pink mass; very 

 scant, white aerial mycelium at margin. 



Ca-agar: Yellowish, wrinkled, coherent 

 growth with white aerial mycelium on apices 

 and at margin. 



Coon's agar: Colorless, mostly submerged 

 growth; scant white aerial mycelium. 



Dorset's egg medium: A few, round, color- 

 less colonies in 3 days; after 3 weeks, irregu- 

 lar, raised, pink mass; warted appearance; 

 moderate degree of liquefaction. 



Serum agar: Raised, convoluted, slightly 

 pinkish growth. 



Inspissated serum: No growth. 



Broth: Moderate quantity of flakes and 

 dust-like surface growth. 



Synthetic sucrose solution: A few color- 

 less flakes on the surface; lesser bottom 

 growth. 



Milk: Yellowish surface growth; solid 

 coagulum in one month; later, partly di- 

 gested, pale pink growth up the wall of the 

 tube. 



Litmus milk: Colorless surface growth; 

 liquid blue; becomes hydrolyzed and de- 

 colorized. 



Potato plug: Small, raised, pale pink 

 colonies with white aerial mycelium; after 

 2 months, plug and liquid discolored, growth 

 dull buff, dry and convoluted at base, round 



and zonate at top of slant, white aerial 

 mycelium, surface and bottom growth on 

 liquid. 



Source : Isolated from a case of mycetoma 

 of the chest wall in a South African native. 



Habitat: Found in human infections so 

 far as known. 



9. Nocardia vaccinii Demaree and 

 Smith, 1952. {Actinomyces sp. Demaree, 

 Phytopath., 37, 1947, 438; Demaree and 

 Smith, Phytopath., 4^, 1952, 249.) 



vac.cin'i.i. M.L. noun Vaccinium generic 

 name of the blueberry; M.L. gen. noun 

 vaccinii of Vaccinium. 



Rods and filaments, 0.4 to 0.8 micron in 

 diameter, granular when stained, eventually 

 breaking up into bacillary forms. A few cells 

 are acid-fast. Presence of fat was demon- 

 strated by staining with Sudan black B. 



Gelatin: Dry, ribbon growth; no hydroly- 

 sis. 



Agar: Poor, slow, granular, gray growth 

 which is sometimes pinkish in old cultures. 



Synthetic agar: Scant, gray growth. 



Starch agar: Dry, ribbon, pinkish to 

 orange growth. 



Potato-yeast-mannitol agar: Abundant, 

 fluffy, gray to orange growth. 



Milk: Dry, raised, gray growth with 

 orange spots. Casein not hydrolyzed. 



Potato: Slow, spreading, raised, gray 

 growth. 



On a basal agar with ammonia as a source 

 of nitrogen, acid was produced from glu- 

 cose, sucrose, glycerol and mannitol; reac- 

 tions variable with arabinose and xylose; 

 no growth on lactose or sorbitol. 



Starch is hydrolyzed. 



Citrates utilized to a limited extent. 



Paraffin is utilized. 



Nitrites slowly produced from nitrates. 



Optimum temperature, between 25° and 

 28° C; inhibited at 32° C; no growth or 

 very scant growth at 37° C. 



Antagonistic properties : None. 



Distinctive characters: Resembles No- 

 cardia 7ninima but diff'ers from it in the 

 following respects: utilizes glycerol and 

 mannitol and sometimes arabinose and 

 xylose; reduces nitrates to nitrites; utilizes 

 citrates and causes formation of bud-pro- 

 liferating galls on blueberry plants. 



