FAMILY II. ACTINOMYCETACEAE 



725 



chromogenesis; nailhead growth in gelatin 

 stab; branching rods and short filaments. 



Source : Seventy-four strains were isolated 

 from soils in Great Britain and Australia. 



Habitat: Soil. 



14. Nocardia globerula (Gray, 1928) 

 Waksman and Henrici, 1948. {Mycobacterium 

 globendum Gray, Proc. Roy. Soc. London, 

 B, 102, 1928, 265; Proactinomijces globerulus 

 Reed, in Manual, 5th ed., 1939, 838; Waks- 

 man and Henrici, in Manual, 6th ed., 1948, 

 903.) 



glo.be'ru.la. L. noun globus a globe; M.L. 

 dim. adj. globerulus globular. 



Original description supplemented by 

 material taken from Bynoe (Thesis, McGill 

 University, Montreal, 1931). 



Curved rods and filaments, 1 by 2 to 9 

 microns, with many coccoid cells, especially 

 in old cultures. Rods and filaments are fre- 

 quently irregularly swollen. Capsules may 

 be present. Not acid-fast. Gram-positive. 



Gelatin: After 19 days, surface colonies 

 irregularly round, 1 to 2 mm in diameter, 

 conve.x, light buff, smooth, shining; edge 

 entire. Deep colonies: Round, with entire 

 edge. 



Gelatin stab: After 8 days, nailhead, ir- 

 regularly round, convex, pinkish white, 

 smooth, shining growth; line of stab erose. 



Agar: After 4 days surface colonies irregu- 

 larly round, 3 to 5 mm in diameter, convex, 

 white, smooth, shining; edge undulate, 

 erose. After 7 daj^s, more convex and of a 

 watery appearance. Deep colonies: After 4 

 days, lens-shaped. 



Agar slant: After 3 days, filiform, flat, 

 watery; edge irregular. 



Dorset's egg medium: After 2 weeks, 

 spreading, raised, moist, orange-colored 

 growth. 



Loefller's medium: Growth as on Dorset's 

 egg medium, but salmon-colored. 



Nutrient and peptone broth: Turbid with 

 viscous suspension. 



Litmus milk: Alkaline. 



Glycerol potato: After 24 hours, filiform, 

 moist, smooth, pale pink growth. 



Indole not produced. 



Indole agar: Blue crystals of indigotin 

 formed. 



No acid from glucose, lactose, maltose, 

 sucrose or glycerol. 



Phenol is utilized. 



Nitrites not produced from nitrates. 



Optimum temperature, between 25° and 

 28° C. 



Optimum pH, between 6.8 and 7.6. 



Distinctive characters: This organism re- 

 sembles most closely Nocardia coralUna. It 

 is distinguished by producing a more watery 

 type of surface growth, more nearly entire 

 deep colonies and more particularly by the 

 production of indigotin from indole. 



Source: Isolated from soil in Great 

 Britain. 



Habitat: Presumably soil. 



15. Nocardia salmonicolor (den Dooren 

 de Jong, 1927) Waksman and Henrici, 1948. 

 {Mycobacterium salmonicolor den Dooren de 

 Jong, Cent. f. Bakt., II Abt., 71, 1927, 216; 

 Proactinomyces salmonicolor Jensen, Proc. 

 Linn. Soc. New So. Wales, 57, 1932, 368; 

 Waksman and Henrici, in Manual, 6th ed., 

 1948, 904; also see Erikson, Jour. Gen. 

 Microbiol., S, 1949, 364.) 



sal.mo.ni'co.lor. L. noun salmo salmon; 

 L. gen. noun salmonis of a salmon; M.L. adj. 

 salmonicolor salmon-colored. 



On glucose-asparagine-agar after 18 to 24 

 hrs., long branching rods are formed, 1.0 to 

 1.3 microns in diameter, with small refrac- 

 tive granules of aerial mycelium, sometimes 

 stretching into quite long filaments; after 

 2 to 3 days small definite mycelia are pres- 

 ent, and after 5 to 6 days these have largely 

 divided into short rods and cocci; the col- 

 onies have the same burr-like appearance as 

 those of Nocardia corallina. Many cells at 

 the edge of the colonies show, after 3 to 4 

 days, club- or pear-shaped swellings, up to 

 2.5 to 3.0 microns in diameter; after 5 to 6 

 days, many of these swollen cells are seen to 

 germinate with the formation of two more 

 slender sprouts. On some media a few short, 

 undivided aerial hyphae appear which may 

 actually form a thin white frosting over the 

 pink growth. Acid-fastness is found among 

 the earlier stages of growth in some of the 

 strains on some media. 



Gelatin: At 20° to 22° C, scant, arbores- 

 cent growth in stab; small, wrinkled orange, 

 surface colony. No liquefaction. 



