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ORDER V. ACTINOMYCETALES 



Dorset's egg medium: Raised, smooth, 

 moist, verrucose, buff -colored growth. 



Loeffler's medium: After 10 daj^s, slight 

 growth, dry, granular, pale buff-colored. 



Broth: Turbid. 



Litmus milk: Slightly alkaline after 5 to 7 

 days. 



Glycerol potato: After 2 days, dry, 

 crumpled, orange, becoming brown after 

 about 10 da3^s. 



No acid from glucose, lactose, sucrose or 

 glycerol. 



Phenol is utilized. 



Nitrites not produced from nitrates. 



Optimum temperature, between 22° and 

 25° C. 



Optimum pH, between 7.6 and 8.0. 



Distinctive characters: Differs from the 

 previously described members of the genus 

 in the absence of chromogenesis. Forms 

 hollow lobes in deep gelatin cultures. Cells 

 are rods, seldom filaments. 



Source : Isolated from soil in Great Britain 

 and Australia. 



Habitat: Presumably soil. 



19. Nocardia transvalensis Pijper and 

 Pullinger, 1927. (Jour. Trop. Med. Hyg., 

 30, 1927, 153.) 



trans. va.len'sis. M.L. adj. transvalensis 

 pertaining to the Transvaal; named for 

 Transvaal, South Africa. 



Description taken from Erikson (Med. 

 Res. Council Spec. Rept. Ser. 203, 1935, 28). 



Initial mycelium unicellular, but with 

 the central branch frequently broader and 

 showing dense, granular refractile contents; 

 small colonies quickly covered with aerial 

 mycelium, the straight aerial hyphae in 

 some cases becoming clustered into irregular 

 spikes; colorless drops are exuded and a pink 

 coloration produced in the densest part of 

 the growth on synthetic glycerol agar. 

 Angular branching with division of the sub- 

 stratum filaments can be seen, the aerial 

 hyphae also being irregularly segmented. 

 Acid-fast. 



Gelatin: Poor growth; there are a few 

 irregular colorless flakes. No liquefaction. 



Agar: No growth. 



Glucose agar: Raised, granular, pink 

 colonies with white aerial mycelium. 



Glycerol agar: Small, pink, coiled masses 

 with thin, white, aerial mycelium. 



Potato agar: No growth. 



Coon's agar: Colorless growth with 

 liberal, white, aerial mycelium. 



Dorset's egg medium: Small, irregularly 

 raised and coiled dull pink mass. 



Serum agar: Very poor growth. 



Inspissated serum: Scant, colorless, flaky 

 growth; later a minute tuft of pale pink, 

 aerial mycelium. 



Broth: Moderate, flaky growth. 



Synthetic sucrose solution : Poor growth, a 

 few flakes on the surface and a few at the 

 bottom. 



Milk: No change. 



Potato plug: Dry, raised, convoluted, 

 pink growth with white aerial mycelium in 

 one month; dull, pink, brittle surface 

 colonies with paler aerial mycelium float- 

 ing coherently on liquid at base in 2 months. 



Starch not hydrolyzed. 



Source: Isolated from a case of mj^ce- 

 toma of the foot in South Africa. 



Habitat: Found in human infections so 

 far as known. 



20. Nocardia mesenterica (Orla-Jen- 

 sen, 1919) Waksman and Henrici, 1948. 

 (Microbacterium mesentericum Orla-Jensen, 

 The Lactic Acid Bacteria, 1919, 181; Pro- 

 actinormjces mesentericus Jensen, Proc. Lin- 

 nean Soc. New So. Wales, 57, 1932, 373; 

 Waksman and Henrici, in Manual, 6th ed., 

 1948, 907.) 



me.sen.te'ri.ca. Gr.noun mesenterium 

 the mesentery; M.L. adj. mesentericus per- 

 taining to the mesentery. 



Extensive mycelium composed of richly 

 branching hyphae of a somewhat variable 

 diameter, 0.4 to 0.8 micron; no aerial 

 hyphae are seen. With increasing age the 

 hyphae divide into fragments of varjang 

 size and shape, partly diphtheroid rods, 

 but no real cocci. There is, particularly in 

 richer media, a tendency to form large, 

 swollen, fusiform to almost spherical cells, 

 up to 3.5 microns in diameter. These may 

 stain intensely with carbol fuchsin; when 

 transferred to fresh media, they germinate 

 and produce a new mycelium. 



Gelatin: Good growth; finely arborescent, 

 cream-colored growth in the stab; raised, 



