FAMILY II. ACTINOMYCETACEAE 



731 



nig'ra. L. adj. niger black. 



Colonies rough, folded, shiny, of dough- 

 like consistency. Growth at first colorless or 

 brownish, gradually beconiing darker and, 

 after 10 to 15 days, dark brown and even 

 black. Pigment, similar to melanin, not 

 soluble in the medium. Hyphae are thread- 

 like, breaking up into rods, 0.7 by 2.0 to 

 10.0 microns, and cocci, 0.6 to 0.8 micron. 

 Aerial mycelium not formed. 



Gelatin: No growth or liquefaction. 



Agar: Poor growth. 



Synthetic agar: Poor growth. Many cells 

 are swollen to 3.0 microns in diameter. 



Broth; Small sediment produced. Medium 

 clear. 



Milk: No change. 



Potato: Good growth. 



Cellulose: No growth. 



Source : Isolated from soil. 



Habitat: Soil. Rarely found. Growth 

 rapidly disappears on continued cultivation. 



27. Nocardia lutea Christopherson and 

 Archibald, 1918. (Lancet, 2, 1918, 847). 



lu'te.a. L. adj. luteus yellow. 



Description taken from Erikson (Med. 

 Res. Council Spec. Rept. Ser. 203, 1935, 30). 



Initial elements swollen and segmented, 

 giving rise to irregular, spreading, polymor- 

 phous colonies composed of cells of all 

 shapes and sizes with markedly granular 

 contents; later more monomorphous, the 

 filaments being arranged in angular apposi- 

 tion. Sometimes (e.g., on synthetic glycerol 

 agar) the segments are so granular as to ap- 

 pear banded. On potato agar, small, fila- 

 mentous colonies are formed with irregular, 

 angular branching, and which bear a few 

 isolated short straight aerial hyphae. 



Gelatin: Pale pink wrinkled growth on 

 wall of tube; colorless, punctiform colonies 

 and stellate colonies are in the medium; no 

 liquefaction. 



Agar: Abundant, coherent, moist, pink, 

 membranous growth with round, discrete 

 colonies at margin; after 3 weeks, colorless, 

 fringed margin, round confluent portion. 



Glucose agar: Scant, reddish, smeary 

 growth. 



Glycerol agar: Yellowish pink, wrinkled 

 membrane. 



Potato agar: Coherent, pink, moist 



growth, centrally embedded with small, 

 round, discrete colonies at margin. 



Dorset's egg medium: Poor growth, dull 

 pink, spreading. 



Serum agar: Confluent, granular, pink 

 membrane. 



Broth: Pink flakes and surface growth. 



Inspissated serum: Raised, convoluted, 

 pink mass; becoming orange and much 

 wrinkled with a scalloped margin. 



Synthetic sucrose solution: Red granules 

 and abundant, minute, colorless colonies at 

 bottom; in 2 weeks a colorless, dust-like 

 surface pellicle. 



Glucose broth: Abundant, pinkish, flaky 

 surface growth, breaking up easily and sink- 

 ing to bottom. 



Potato plug: Carrot-red, moist, thick, 

 granular growth in bands, partly raised, 

 and with discrete round colonies; sparse, 

 colorless, very thin aerial mycelium at top 

 of slant in 2 months. 



Litmus milk: Orange-red surface and 

 bottom growth; liquid blue. 



Source: Isolated from actinomycosis of 

 the lachrymal gland. 



Habitat: Unknown, 



28. Nocardia blackwellii (Erikson, 1935) 

 Waksman and Henrici, 1948. {Actinomyces 

 blackwellii Erikson, Med. Res. Council 

 Spec. Rept. Ser. 203, 1935, 37; Waksman and 

 Henrici, in Manual, 6th ed., 1948, 910.) 



black. wel'li.i. M.L. gen. noun blackwellii 

 of Blackwell; named for Blackwell. 



Description taken from Erikson (op. cit., 

 1935, 32). 



Initial elements short and rod-like, grow- 

 ing out into sparsely branching longer 

 forms; small, radiating colonies are pro- 

 duced with short, straight aerial mycelia; 

 frequently large, round or ovoid cells are 

 interposed in the irregularly segmented 

 chains of cells, being sometimes isolated 

 in company with 2 or 3 short filaments and 

 sometimes terminal. 



Gelatin: Few, colorless, minute colonies 

 along line of inoculation; after 30 days 

 abundant, colorless colonies to 10 mm be- 

 low surface, larger pink-yellow surface 

 colonies with white aerial mycelium; no 

 liquefaction. 



Agar: Confluent, wrinkled growth with 



