732 



ORDER V. ACTINOMYCETALES 



small, round, pinkish, discrete colonies at 

 margin. 



Glucose agar: Abundant, pale pink 

 growth; small conical colonies, piled up, 

 convoluted. 



Glycerol agar: Extensive, granular, ir- 

 regular, thin, pinkish growth; after 40 

 days, a few discrete colonies with de- 

 pressed margins, center piled up, pink. 



Serum agar: Smooth, cream, umbilicated 

 colonies with submerged growth extending 

 into medium in scallops 5 to 8 mm deep; a 

 pale pink mass in 2 weeks. 



Potato agar: Small, round, colorless 

 colonies covered with white aerial my- 

 celium; after 2 weeks colonies dull pink; 

 submerged margins; few aerial spikes; 

 moderate aerial mycelium at top of slant. 



Broth: Flakes, later innumerable, minute 

 colonies, some adhering to wall just above 

 liquid level. 



Synthetic sucrose solution: Delicate, 

 round, white colonies; later abundant 

 minute colonies in suspension; thick cream 

 pellicle on surface and pink grains in sedi- 

 ment. 



Milk: Heavy, convoluted, bright yellow 

 surface pellicle; no coagulation. 



Litmus milk: Yellow surface growth; 

 milky sediment; liquid unchanged. 



Carrot plug: Small, round, smooth, 

 cream-colored, elevated colonies in 10 days; 

 sparse, stiff, colorless aerial spikes in 16 

 days; abundantly piled up, convoluted, 

 ochreous growth in 25 days. 



Source: Isolated from the hock joint of a 

 foal. 



Habitat: Unknown. 



29. Nocardia cuniculi Snijders, 1924. 

 (Geneesk. Tijdsch. Ned. Ind., 6^, 1924, 47 

 and 75.) 



cu.ni'cu.li. L. noun cuniculus a rabbit; 

 L. gen. noun cuniculi of a rabbit. 



Description taken from Erikson (Med. 

 Res. Council Spec. Rept. Ser. 203, 1935, 31). 



Large, swollen cells giving rise to ramify- 

 ing filaments or to small chains of short, 

 thick segments which branch out into 

 more regular hyphae; sometimes the ir- 

 regular elements are beset with spiny proc- 

 esses before giving rise to typical long 

 branching filaments; later the picture be- 



comes more monomorphous, and short, 

 straight aerial hyphae are borne which pres- 

 ently exhibit irregular segmentation. 



Gelatin: Few flakes. No liquefaction. 



Agar: Small, round, elevated, cream - 

 colored colonies, umbilicated and radially 

 wrinkled. 



Glucose agar: Minute, colorless colonies; 

 becoming dull pink, partly confluent and 

 piled up; few stiff pink aerial spikes. 



Glycerol agar: Small, round, elevated, 

 cream-colored colonies; margins depressed; 

 becoming smooth, discrete, yellowish. 



Dorset's egg medium: Scant, pinkish, 

 smeary growth. 



Serum agar: Small, raised, cream-colored 

 colonies, becoming confluent and piled up. 



Inspissated serum: Thick, colorless, 

 ribbed membrane; no liquefaction. 



Broth: Small and larger cream-colored, 

 scale-like surface colonies; abundant, 

 flocculent bottom growth. 



Synthetic sucrose solution: Thin surface 

 pellicle; small colorless flakes; minute par- 

 ticles at bottom; scant growth. 



Milk: Heavy yellow growth attached to 

 walls; solid coagulum in 1 month. 



Litmus milk: Yellow surface growth; 

 liquid unchanged. 



Potato plug: Coral -pink, dry, granular 

 growth covered to a considerable extent 

 with white aerial mycelium, piled up in 

 center, discrete colonies at margin, pink 

 surface pellicle on liquid and colorless 

 colonies at base. 



Source: Isolated from infected rabbits. 



Habitat: Unknown. 



30. Nocardia rangoonensis (Erikson, 

 1935) Waksman and Henrici, 1948. (Actino- 

 myces rangoon Erikson, Med. Res. Council 

 Spec. Rept. Ser. 203, 1935, 37; Waksman 

 and Henrici, in Manual, 6th ed., 1948, 911.) 



ran. goo. nen 'sis. M.L. adj. rangoonensis 

 pertaining to Rangoon; named for Rangoon, 

 Burma. 



Swollen, round initial cells, giving rise 

 to branching hyphae which segment and 

 present slipping and angular arrangement; 

 few short straight aerial hyphae, which later 

 develop into a profusely branching, long 

 waving aerial mycelium. Non-acid-fast. 



