FAMILY II. ACTINOMYCETACEAE 



733 



Gelatin: Abundant, minute colonies in 

 depths and larger cream-colored ones on 

 surface with white aerial mycelium; brown 

 pigment surrounding growth. No liquefac- 

 tion. 



Agar colones: Round, lobate, umbili- 

 cated, raised up, cream-colored to pale 

 pink; later, medium discolored dark brown, 

 colonies colorless. 



Glucose agar: Convoluted, coherent, 

 cream-colored growth; medium discolored. 

 After 23 days, wrinkled, biscuit-colored 

 growth; colorless margin; border white 

 aerial mycelium; medium dark brown. 



Glycerol agar: Dull, mealy, pink, 

 wrinkled growth; scant white aerial myce- 

 lium at top; medium slightly discolored. 



Coon's agar: Minute, colorless colonies in 

 streaks. 



Potato agar: Small, round, lemon-colored 

 colonies, partly confluent, with white aerial 

 mycelium; later medium discolored light 

 brown; submerged growth greenish. 



Dorset's egg medium: Extensive, color- 

 less growth, pale pink aerial mycelium in 

 center; later covered with a powdery, pink- 

 ish white aerial mycelium. 



Serum agar colonies: Irregular, small, 

 elevated, cream-colored, frequently um- 

 bilicated. 



Inspissated serum: Poor growth; small 

 piled up pink mass. 



Broth: Abundant colorless growth; floc- 

 culent mass at bottom and pellicle at sur- 

 face; medium slightly discolored. 



Synthetic sucrose solution: Small, white 

 colonies with pinkish tinge on surface; 

 lesser bottom growth. 



Milk: Coagulation; yellow surface ring; 

 becoming partly peptonized; liquid dis- 

 colored dark brown; brownish growth up 

 side of tube. 



Litmus milk: Colorless growth; liquid 

 partly decolorized; coagulation; later partly 

 digested. 



Carrot plug: Small, round, colorless colo- 

 nies; velvety white aerial mycelium; in 2 

 months, piled up, pink, granular mass 

 with warted prominences; marginal zone 

 white aerial mycelium and thin all-over 

 central aerial mycelium. 



Source : Isolated from a human pulmonary 

 case of streptothricosis. 



Habitat: Unknown. 



31. Nocardia caviae Snijders, 1924. 

 (Geneesk. Tijdschr. Ned. Ind., 6^, 1924, 47 



and 75.) 



ca'vi.ae. M.L. noun Cavia generic name 

 of the cavy, or guinea pig. 



Description taken from Erikson (Med. 

 Res. Council Spec. Rept. Ser. 203, 1935, 32). 



Initial segmentation, producing elements 

 of approximately even diameter arranged 

 in angular apposition, and later long pro- 

 fusely ramifying threads with strongly re- 

 fractile protoplasm. Aerial mycelium 

 straight and branching, the aerial hyphae 

 with occasional coiled tips divided into 

 cylindrical conidia. 



Gelatin: A few colorless flakes. No lique- 

 faction. 



Glucose agar: Piled up, convoluted, 

 cream-colored to pale pink growth; white 

 aerial mycelium. 



Glycerol agar: Scant growth. 



Coon's agar: Colorless, scant growth, 

 partly submerged, white aerial mycelium. 



Potato agar: Colorless, spreading growth 

 with dense white aerial mycelium. 



Dorset's egg medium: Heavily corru- 

 gated pale pink growth with submerged 

 margin and dense white aerial mycelium 

 in center; after 3 weeks, colorless, transpired 

 drops. 



Serum agar: Pale pink, wrinkled growth, 

 partly submerged; after 4 weeks, piled up 

 with scant white aerial mycelium; medium 

 discolored reddish brown. 



Inspissated serum: Pale pink, raised 

 growth; coiled, white aerial mycelium. 



Broth: Cream-colored, wrinkled surface 

 pellicle extending up wall and breaking 

 easily; moderate bottom growth, flaky; 

 medium discolored. 



Synthetic sucrose solution: Round, white 

 colonies in suspension and attached to one 

 side of tube; pink surface colonies with 

 white aerial mycelium. 



Milk: Colorless surface growth; white 

 aerial mycelium; coagulation. 



Litmus milk: Liquid blue, surface growth; 

 after 1 month, white aerial mycelium, color- 

 less sediment, liquid still blue. 



Potato plug: Small, colorless colonies; 



