736 



ORDER V. ACTINOMYCETALES 



irregularly wrinkled, with small discrete 

 colonies; clear hemolytic zone. 



Broth: Sediment of flocculi; some round 

 and fan-shaped colonies. 



Synthetic sucrose solution: Very delicate, 

 white flocculi. 



Milk: Coagulated; partly peptonized. 



Potato plug : No growth. 



Starch not hydrolyzed. 



Tyrosine agar: Negative reaction. 



Source : Isolated from the spleen in a case 

 of acholuric jaundice. Injected into a 

 monkey from which it was then reisolated. 



Habitat: Found in human infections so 

 far as known. 



36. Nocardia fructifera (Krassilnikov, 

 1941) Waksman, 1953. (Proactinomyces fruc- 

 tiferi (sic) Krassilnikov, Guide to the Acti- 

 nomycetes, Izd. Akad. Nauk, U.S.S.R., 

 Moskau, 1941, 78; Waksman, in Waksman 

 and Lechevalier, Actinomycetes and Their 

 Antibiotics, Baltimore, 1953, 155.) 



fruc.ti'fe.ra. L. part. adj. fructifenis 

 fruit-bearing. 



Mycelium septate, hyphae breaking up 

 into rods and in some cultures into cocci. 

 Aerial mycelium well developed, whitish 

 to rose-colored. Sporophores long. Straight 

 or weakly wavy, but not spiral-shaped. 

 Oidiospores cylindrical, elongated, 0.7 by 

 1.5 microns. Not acid-fast. Gram-positive. 



Colonies not compact, mostly dough-like 

 in consistency, smooth or rough. 



Gelatin: Slow liquefaction. 



Agar: Aerial mycelium weakly developed 

 or entirely absent. 



Synthetic agar: Rose-colored to bright 

 red and even red-orange growth. Pigment 

 not soluble in medium. 



Milk: Coagulated and weakly peptonized. 



Sucrose is inverted. 



Starch weakly hydrolyzed. 



Cellulose: Poor growth. 



Fats: Good growth. 



Comment: This species is considered as a 

 typical transition point between Strepto- 

 myces ruber and Nocardia rubra (Krassilni- 

 kov, op. cit., 1941, 78). 



Source: One strain was obtained as a mu- 

 tant of Nocardia rubra. Another strain was 

 changed, after 8 months of cultivation, into 

 a typical Streptomyces. 



Habitat: Unknown. 



37. Nocardia africana Pijper and Pul- 

 linger, 1927. (Pijper and Pullinger, Jour. 

 Trop. Med. and Hyg., 30, 1927, 153; Strep- 

 tomyces africanus Waksman and Henrici, in 

 Manual, 6th ed., 1948, 959.) 



af.ri.ca'na. L. adj. africanus pertaining 

 to Africa. 



Description taken from Erikson (Med. 



Res. Council Spec. Rept. Ser. 203, 1935, 18). 



Unicellular, branching mycelium forming 



small, dense, pink colonies with short, 



straight, sparse, white aerial mycelium. 



Gelatin: Irregular pink flakes. No lique- 

 faction. 

 Agar: A few, flat, pink, discoid colonies. 

 Glucose agar: Minute, red, discrete, round 

 colonies and piled up, paler pink mass with 

 thin white aerial mycelium. 



Glycerol agar: After 2 weeks, small, 

 heaped-up, colorless masses with pink tinge 

 around the colorless colonies; margin de- 

 pressed; after 3 weeks, abundant, piled up, 

 pale pink growth. 



Ca-agar: After 1 week, small, round, 

 colorless colonies with red centers ; margins 

 submerged; after 2 weeks, growth bright 

 cherry-red, confluent, with colorless margin. 

 Dorset's egg medium: Small, colorless 

 blister colonies, partly confluent; become 

 wrinkled and depressed into medium; slight 

 liquefaction. 



Serum agar: Irregularly round, raised, 

 wrinkled, colorless colonies; becoming dry, 

 pink and flaky; later piled up, brownish, 

 friable. 



Inspissated serum: After one week, 

 smooth, round, colorless colonies with sub- 

 merged margin, in confluent patches pink 

 and pitted into medium; after 2 weeks, me- 

 dium broken up, slight liquefaction; after 3 

 weeks, liquid dried up, colonies umbilicated, 

 raised, dry and friable. 



Broth: Small pink colonies embedded in 

 coherent flocculent mass. 



Synthetic sucrose solution: Small pink 

 granules in sediment after 1 week; colonies 

 of medium size, coherent, after 3 weeks. 



Potato agar: Bright red growth, small 

 round colonies with colorless submerged 

 margins and piled up patches with stiff', 

 sparse, white aerial mycelium. 



Litmus milk: Bright red surface growth; 

 liquid unchanged after one month; liquid 



