FAMILY II. ACTINOMYCETACEAE 



737 



opaque reddish purple after 2 months; hy- 

 drolyzed, clear wine-red after 3 months. 



Antagonistic properties: Positive. 



Source : Isolated from a case of mycetoma 

 of a foot in South Africa. 



Habitat: Unknown. 



38. Nocardia pelletieri (Laveran, 1906) 

 Pinoy, 1912. (Micrococcus pelleiieri Lixvemn, 

 Compt. rend. Soc. Biol., Paris, 61, 1906, 340; 

 Oospora pelletieri Thirou.x and Pelletier, 

 Bull. Soc. path, exot., 5, 1912, 588; Pinoy, 

 in Thiroux and Pelletier, ibid., 589; Strep- 

 tomyces pelletieri Waksman and Henrici, in 

 Manual, 6th ed., 1948, 960.) 



pel.le.ti.e'ri. M.L. gen. noun pelletieri of 

 Pelletier; named for M. Pelletier, the first to 

 isolate this species. 



Description taken from Erikson (Med. 

 Res. Council Spec. Rept. Ser. 203, 1935, 21). 



Mycelium composed of slender, straight 

 and not very long filaments, forming small, 

 dense, pink colonies with a few short, 

 straight, isolated aerial branches. 



Gelatin: Slight liquefaction; few pink 

 flakes; later almost completely liquefied. 



Agar: Minute, colorless colonies and 

 piled-up, pale pink masses. 



Glucose agar: Poor growth; a few minute 

 pink colonies. 



Glj^cerol agar: Poor growth; a few moist 

 pink colonies. 



Ca-agar: Colorless, small colonies; after 

 1 week, confluent skin, pink, buckled; me- 

 dium discolored later. 



Coon's agar: Poor growth, cream-colored 

 with pink center, mostly submerged. 



Potato agar: Colorless blister colonies; 

 after 3 weeks, colonies larger, showing con- 

 centric zones, submerged margins and oc- 

 casional zone or tuft of white aerial my- 

 celium, pinkish coloration. 



Dorset's egg medium: Abundant, wrin- 

 kled, pink skin with small discrete colonies 

 at margin in six days; later surface rough, 

 mealy; considerable liquefaction in 17 days. 



Serum agar: Moist, cream-colored growth 

 tending to be heaped up; discrete colonies 

 at margin; becoming umbilicated. 



Inspissated serum: Round, moist, color- 

 less colonies. 



Blood agar: At first a few pinhead, cream- 

 colored colonies, no hemolysis; later col- 



onies dense, button-shaped, with narrow 

 fringed margin. 



Broth: Small, minute, pink, clustered 

 colonies. 



Synthetic sucrose solution: Small, pink 

 colonies in sediment; later minute colonies 

 adhering to side of tube. 



Milk: Soft curd; half -digested; peptoniza- 

 tion complete in 20 days. 



Litmus milk: Pink surface growth, semi- 

 solid, no color change; after 20 days, coagu- 

 lum cleared, liquid purple. 



Potato plug: After one month growth 

 sparse, yellowish pink, irregularly piled up, 

 portions with scant white aerial mycelium; 

 after 6 months abundant, highly piled-up, 

 small, rounded pink masses; scant white 

 aerial mycelium persistent. 



Relationships to other species: Thiroux 

 and Pelletier (Bull. Soc. path, exot., 5, 1912, 

 585) considered that their cultures resem- 

 bled Nocardia madtirae, but they grew the 

 organism only on Sabouraud's gelatin, on 

 which it appeared in a constantly red, easily 

 detachable form. Nocardia indica was re- 

 garded as identical by Pinoy, although in 

 the original description by Laveran the 

 organism was called Micrococcus pelletieri, 

 owing to the fact that no mycelium was 

 seen, merely coccoid bodies. Nocardia 

 genesii Froes (Bull. Inst. Past., 29, 1931, 

 1158) is described as closely allied, the dis- 

 tinction being founded upon the fact that 

 the red grains were smaller in size and much 

 more numerous, but no cultural details are 

 given. 



Source: Isolated from a case of crimson- 

 grained mycetoma in Nigeria (E. C. Smith, 

 Trans. R. Soc. Trop. Med. Hyg., 22, 1928, 

 157). 



Habitat: L^nkiiown. 



39. Nocardia niaculata (Millard and 

 Burr, 1926) Waksman and Henrici, 1948. 

 {Actinomyces maculatus Millard and Burr, 

 Ann. Appl. Biol., 13, 1936, 580; Proactino- 

 myces maculatus Umbreit, Jour. Bact., 38, 

 1939, 84; Waksman and Henrici, in Manual, 

 6th ed., 1948, 913.) 



ma.cu.Ia'ta. L. i)art.adj. tnaculatus 

 spotted. 



Filamentous organisms possessing a 

 ough, shiny colony which is cartilaginous. 



