FAMILY II. ACTINOMYCETACEAE 



739 



branching mycelium. Black pigment slowly 

 produced. 



Litmus milk: Unchanged. 



Potato: Barnacle-like, brownish, spread- 

 ing growth; no aerial mycelium. Medium 

 brownish around growth. 



Indole not produced. 



No acid from glucose, lactose, maltose, 

 mannitol, sucrose or dulcitol. 



Good growth at 25° C. Slow growth at 

 37° C. 



Distinctive character: Produces an anti- 

 biotic substance (proactinomycin) upon 

 synthetic and organic media which is pri- 

 marily active against various Gram-positive 

 bacteria. 



Source: Isolated as an air contaminant at 

 Oxford, England. 



Habitat: Unknown. 



42. Nocardia fordii (Erikson, 1935) 

 Waksman, 1953. (Actinomyces fordii Erik- 

 son, Med. Res. Council Spec. Rept. Ser. 203, 

 1935, 15 and 36 ; Streptomyces fordii Waksman 

 and Henrici, in Manual, 6th ed., 1948, 958; 

 Waksman, in Waksman and Lechevalier, 

 Actinomycetes and Their Antibiotics, Bal- 

 timore, 1953, 159.) 



for'di.i. M.L. gen. noun fordii of Ford; 

 presumably named for the surgeon who first 

 secured the culture. 



Filaments of medium length, no spirals or 

 markedly wavy branches. Short, straight, 

 sparse aerial mycelium. Small ovoid conidia 

 on potato agar and starch agar. 



Gelatin : No visible growth, slight soften- 

 ing in 20 daj^s; half-liquefied after 40 days. 



Agar: Small, creamy golden, ring-shaped 

 colonies and heaped-up patches, becoming 

 golden brown in color and convoluted. 



Glycerol agar: Extensive, golden brown, 

 convoluted, thin layer. 



Serum agar: Golden brown, ring-shaped 

 and coiled smooth colonies; no liquefaction. 



Ca-agar: Yellow, scale-like, closely ad- 

 herent colonies; scattered white aerial my- 

 celium. 



Blood agar: Innumerable, small, yellow- 

 ish, ring-shaped colonies; no hemolysis. 



Broth: Few flakes at first; later abundant, 

 coherent, puffball growth. 



Synthetic sucrose solution: Moderate 

 sediment of minute round white colonies. 



Synthetic glycerol solution: Light white 

 fluffy colonies, minute and in clusters. 



Inspissated serum: Innumerable, color- 

 less, pinpoint colonies; scant white aerial 

 mycelium; after 15 d&ys colonies large, 

 hollow on reverse side; margin depressed; 

 no liquefaction. 



Dorset's egg medium: Minute, cream- 

 colored, elevated colonies, becoming golden 

 brown, raised, convoluted. 



Milk: Coagulated; brownish surface ring. 



Litmus milk: No change in reaction. 



Potato plug: Yellowish growth in thin 

 line, terminal portion tending to be piled 

 up; scant white aerial mycelium at top of 

 slant; after 12 days, growth abundant, 

 golden brown, confluent, partly honey- 

 combed, partly piled up. 



Starch not hydrolyzed. 



Tyrosine agar: Reaction negative. 



Source: Isolated from a human spleen in a 

 case of acholuric jaundice. 



Habitat: Unknown. 



43. Nocardia kuroishi Uesaka, 1952. 

 (Jour. Antibiotics (Japanese), 5, 1952, 75.) 



ku.ro. i'shi. Etymology Japanese, mean- 

 ing uncertain. 



Mycelium gives a weak acid-fast reaction, 

 but the separated cells, 0.8 by 1.3 microns, 

 are not acid-fast. 



Aerial mycelium: Abundant, branching 

 hyphae slightly curved at first, later turning 

 around each other; cells refractive. Gran- 

 ules soon become visible. 



Gelatin: Yellowish brown growth sinking 

 into medium. No aerial mycelium. Yellowish 

 brown, soluble pigment. No liquefaction. 



Synthetic glycerol agar: Thin, pale yellow 

 growth, partly covered with punctiform, 

 white aerial mycelia. Yellow pigment. 



Agar: Wrinkled, grayish yellow colonies. 

 No aerial mycelium. Faint, grayish brown, 

 soluble pigment. 



Glucose agar: Abundant growth, at first 

 yellowish brown then reddish brown. Scant 

 white aerial mycelium at margin of colonies. 

 Red to wine-colored, soluble pigment. 



Synthetic solution: White, minute 

 colonies on surface. Medium becomes 

 brown. 



Glucose broth: Red colonies, forming a 



