FAMILY III. STREPTOMYCETACEAE 



755 



milk coagulation, proteolysis and pigmenta- 

 tion of aerial mycelium. 



Source: Isolated from soil. 



Habitat: Soil. 



4. Streptoniyces anulatus (Beijerinck, 

 1912, emend. Krassilnikov, 1941) Waksman, 

 1953. (Streptothrix annulatus (sic) Beijerinck, 

 Folia Microbiologica, 1, 1912, 4; Actinomyces 

 annulatus (sic) Krassilnikov, Guide to the 

 Actinomycetes, Izd. Akad. Nauk, U.S.S.R., 

 Moskau, 1941, 40; Waksman, in Waksman 

 and Lechevalier, Actinomycetes and Their 

 Antibiotics, Baltimore, 1953, 40.) 



a.nu.la'tus. L. adj. anulatus furnished 

 with a ring. 



Aerial mycelium: Whitish or light gray. 

 Sphorophores produce spirals with 3 to 7 

 turns (sinistrorse) ; spores spherical, 0.7 

 micron in diameter. 



Gelatin: Slow liquefaction. 



Agar: White aerial mycelium, concentric 

 rings less marked than those on synthetic 

 agar. 



Synthetic agar: Colorless, flat growth, 

 penetrating deep into agar. White, velvety 

 aerial mycelium growing in the form of 

 concentric rings. 



Milk: Coagulated, slowly peptonized. 



Sucrose is inverted. 



Starch is hj^drolyzed. 



Good growth on cellulose. 



Odor: Strong, earthy. 



Antagonistic properties: Highly antago- 

 nistic against mycobacteria and Gram- 

 positive bacteria; some strains are active 

 against fungi. 



Source: Isolated from soil. 



Habitat: Soil. 



5. Streptoniyces rochei Waksman, 1953. 

 {Streptoniyces rochei Berger, Jampolsky and 

 Goldberg (nomen nudum), Arch, of Bio- 

 chem., 22, 1949, 477; Waksman, in Waksman 

 and Lechevalier, Actinomycetes and Their 

 Antibiotics, Baltimore, 1953, 40.) 



ro'che.i. M.L. gen. noun rochei of Roche. 



Description prepared by Julius Berger for 

 use in Waksman and Lechevalier, Actino- 

 mycetes and Their Antibiotics, Baltimore, 

 1953. 



Vegetative growth: Vegetative mycelium 

 characteristically fine, 0.8 to 1.5 microns in 



diameter, with short branches on synthetic 

 (calcium malate) and non-synthetic (pep- 

 tone-tomato paste) agars. 



Aerial mycelium: Sporogenous hyphae 

 1.5 microns in diameter, often spirally 

 twisted; spirals usually short and loose with 

 rarely more than 2 to 3 coils. Spores ellip- 

 soidal, sometimes spherical, 0.8 to 1.5 by 

 1.3 to 2.8 microns. 



Gelatin: Cream-colored surface ring, 

 covered with white aerial mycelium. Rapid 

 liquefaction. Faint yellow soluble pigment. 



Agar: Cream-colored growth. White aerial 

 mycelium. No soluble pigment. 



Synthetic agar: Thin, colorless growth, 

 covered with sandy lavender to dark gray 

 aerial mycelium. Reverse light gray, later 

 becoming grayish yellow. No soluble pig- 

 ment. 



Ca-malate-glycerol agar: Abundant 

 growth, raised in center. Gray aerial myce- 

 lium, buff around the edges, having a fuzzy 

 appearance. Medium is cleared directly 

 under the growth. 



Starch agar: Brownish growth. Mouse- 

 gray aerial mycelium. Reverse shows slight 

 purple pigmentation in four days. 



Glucose agar: Smooth, yellowish growth, 

 covered with white to gray aerial mycelium. 

 Yellowish soluble pigment. 



Ca-citrate-glycerol broth: Growth at 

 surface forming a thin mat of partially 

 sporulated, discrete, grayish white colonies. 

 From the mat, soft, round, fuzzy, gray colo- 

 nies drop into the medium. 



Milk: Ring at the surface cream-colored 

 to brownish. Coagulated; rapidly pepto- 

 nized. 



Potato: Abundant, lichenoid, cream-col- 

 ored growth. Abundant, cottony, white to 

 gray aerial mycelium. Color of plug becomes 

 reddish tan. 



Strong diastatic action. 



Antagonistic properties: On certain com- 

 plex nitrogenous media such as those con- 

 taining soybean flour or distillers' dried 

 solubles, the organism produces a wide 

 range of antimicrobial activity. Part of 

 this is attributable to a specific antibiotic 

 known as borrelidin. 



Comment": Morphologically the culture 

 resembles some species in the Streptomyces 

 albus group, such as Streptomyces alhido- 



