FAMILY III. STREPTOMYCETACEAE 



763 



growth, partly covered with white mycelium 

 on surface of medium; soluble yellow-ochre 

 pigment. 



Milk: Rapid growth becoming covered 

 with whitish aerial mycelium; never fully 

 covering the surface; no coagulation; pep- 

 tonization begins slowly and is completed 

 in 13 days, liquid becoming colored yellow- 

 ish orange. 



Coagulated serum: Cream-colored growth 

 of surface becoming covered with white 

 aerial mj-celium; rapid liquefaction of 

 serum. 



Starch medium: Cream-colored growth 

 rapidly colored with yellow aerial myce- 

 lium; after 20 days growth becomes much 

 folded; greenish on reverse side; slightly 

 amber-colored in medium. 



Antagonistic properties: Positive. 



Comments: This species is closely related 

 to Streptomyces albus. Develops poorly on 

 synthetic media without asparagine. 



Source: Isolated from dust. 



Habitat: Unknown. 



23. Streptomyces lieskei (Duche, 1934) 

 Waksman and Henrici, 1948. {Actinomyces 

 lieskei Duch6, Encyclop^die Mycologique, 

 Paris, 6, 1934, 289; Waksman and Henrici, 

 in Manual, 6th ed., 1948, 950.) 



lies'ke.i. M.L. gen. noun lieskei of Lieske; 

 named for Prof. Rudolf Lieske of Leipzig. 



Gelatin: Cream-colored growth becoming 

 covered with white aerial mycelium; no 

 soluble pigment. Rapid liquefaction. 



Agar: Cream-colored growth becoming 

 covered with white aerial mycelium; yel- 

 lowish soluble pigment. 



Synthetic agar: Cream-colored growth 

 with delayed white aerial mycelium grow- 

 ing from the edge toward the center; my- 

 celium later j^ellowish. Reverse of growth 

 yellowish to green. Dirty yellow to yellow- 

 green soluble pigment. 



Synthetic solution: Long branching fila- 

 ments 0.7 micron in diameter. Yellowish 

 white aerial mycelium does not readily 

 produce spores; flakes drop to the bottom 

 of the tube. 



Peptone solution: Cream-colored colo- 

 nies on surface with flakes in the liquid 

 dropping to the bottom of the tube. Liquid 

 becomes yellowish in color. 



Tyrosine medium: Rapid growth on sur- 

 face with whitish yellow aerial mycelium; 

 yellowish to orange-yellow soluble pigment. 



Milk: Cream-colored growth; colorless on 

 reverse side; no aerial mycelium. Peptoniza- 

 tion without coagulation. After 20 days the 

 whole milk becomes a clear yellowish liquid. 



Coagulated serum: Clear-colored growth. 

 Rapid liquefaction. 



Relationship to other species: Culture 

 related to Streptomyces alboflavus and Strep- 

 tomyces albidoflavus . 



Source: Culture secured from the collec- 

 tion of Prof. R. Lieske. 



Habitat: Unknown. 



24. Streptomyces flavovirens (Waks- 

 man, 1923) Waksman and Henrici, 1948. 

 (Actinomyces 128, Waksman, Soil Sci., 8, 

 1919, 117; Actinomyces flavovirens Waksman, 

 in Manual, 1st ed., 1923, 352; Waksman and 

 Henrici, in Manual, 6th ed., 1948, 940.) 



fla.vo.vi'rens. L. adj. flavus yellow; L. 

 p. adj. virens being green; M.L. adj. flavo- 

 virens being yellow-green. 



Aerial mycelium: Large masses of minute 

 tufts; the hyphae coarse, straight, short, 

 relatively unbranched, beaded; open spirals 

 may be produced in certain substances. 

 Conidia spherical, ellipsoidal to rod-shaped, 

 0.75 to 1.0 by 1.0 to 1.5 microns. 



Gelatin stab: Yellowish green surface 

 pellicle, consisting of a mass of small colo- 

 nies, on the liquefied medium. 



Agar: Yellowish growth; the reverse dark 

 in center with yellowish zone and outer 

 white zone. 



Synthetic agar: Growth spreading deep 

 into the substratum, yellowish with green- 

 ish tinge. Aerial mycelium gray, powdery. 



Starch agar: Greenish yellow, spreading 

 growth, developing deep into the medium. 



Glucose agar: Restricted growth, de- 

 veloping only to a very small extent into 

 the medium, yellow, turning black, edge 

 entire. 



Glucose broth: Thick, sulfur-yellow pel- 

 licle or ring. 



Litmus milk: Cream-colored to brownish 

 ring; coagulated; peptonized, becoming 

 faintly alkaline. 



Potato: Sulfur-yellow, wrinkled growth. 



Starch is hydrolyzed. 



