^64 



ORDER V. ACTINOMYCETALES 



Greenish yellow soluble pigment formed. 



Only a trace of nitrite is produced from 

 nitrates. 



Aerobic. 



Optimum temperature, 25° C. 



Antagonistic properties: Active against 

 fungi. 



Source: Isolated from soil. 



Habitat: Soil. 



25. Streptoniyces celluloflavus Nishi- 

 mura et al., 1953. (Nishimura, Kimura and 

 Kuroya, Jour. Antibiotics (Japanese), 6, 

 1953, 57.) 



cel.lu.lo.fla'vus. L. dim.n. cellula a small 

 room; M.L. n. cellulosutn cellulose; L. adj. 

 flavus yellow; M.L. adj. celluloflavus cellu- 

 lose-yellow (probably intended to mean 

 turning cellulose yellow). 



Aerial mycelium: A few imperfect spirals. 

 Almost spherical spores, 0.9 by 1.0 micron. 



Gelatin: Ivory-j-ellow to olive-buff colo- 

 nies on surface of liquefied portion. No 

 aerial mycelium. Faint brownish pigment. 

 Rapid to medium liquefaction. 



Agar: Olive-buff growth turning colorless. 

 Scant, cottony, white to grayish aerial 

 mycelium. Soluble yellow pigment with 

 tinge of green to old gold. 



Synthetic agar: Glossy growth, develop- 

 ing deep into medium, later becoming mar- 

 guerite-yellow. Faint sulfur-yellow soluble 

 pigment. 



Ca-malate glycerol agar: Primrose-yellow 

 growth, later turning white to pale olive- 

 buff, with blackish center. Cottony aerial 

 mycelium white with grayish patches, later 

 turning olive-buff. Soluble citron-j-ellow 

 pigment. 



Glucose agar: Cream-colored to yellow 

 growth. Scant cottony white to gray aerial 

 m3^celium. Soluble sulfur-yellow pigment. 



Milk: Yellow to dark olive-buff growth. 

 White aerial mycelium. Soluble reddish 

 brown pigment. Coagulated and rapidly 

 peptonized. 



Potato: Wrinkled, spreading, deep olive- 

 buff growth. Aerial mycelium white to olive- 

 buff. Soluble deep olive-buff pigment. 



Poor growth on cellulose with soluble, 

 yellow pigment. 



Tyrosine medium: Ivory-yellow to cream- 

 buff growth. None to scant white aerial 

 mycelium. Soluble greenish yellow pigment. 



Antagonistic properties: Produces thiolu- 

 tin. 



Source: Isolated from soil. 

 Habitat: Soil. 



26. Streptoniyces limosus Lindenbein, 

 1952. (Arch. f. Mikrobiol., 17, 1952, 379.) 



li.mo'sus. L. adj. limosus full of slime, 

 slimy. 



Vegetative growth: Crumb-like, yellow- 

 ish, later becoming gray to coal-black; 

 greenish yellow reverse. 



Aerial mycelium: Powdery snow-white to 

 gray, or completely lacking. Soluble green- 

 ish yellow to citron-yellow pigment. 



Gelatin: Yellow-brown growth. No aerial 

 mycelium. Soluble, dark brown pigment. 

 Complete liquefaction. 



Agar: Diffuse, colorless growth with light 

 brown reverse. No aerial mycelium. Soluble 

 pigment around bottom. 



Synthetic agar: Diffuse, colorless growth, 

 later becoming crumb-like, light yellow. No 

 aerial mycelium. Soluble citron-yellow pig- 

 ment. 



Glucose asparagine agar: Crumb-like, | 

 light yellow growth, later becoming black 

 with citron-yellow reverse. Aerial mycelium 

 white, later ash-gray. Soluble citron-yellow 

 pigment. 



Ca-malate agar: Crumb-like growth with 

 dark yellow reverse. Aerial mycelium pow- 

 dery to velvety, white, later ash-gray. Solu- 

 ble golden yellow pigment. 



Starch agar: Small, yellowish colonies 

 with brownish reverse. Aerial mj'celium 

 velvety, gray-white. Soluble light gray pig- 

 ment. 



Glucose agar: Good growth with yellow- 

 brown reverse. Aerial mycelium velvet}', 

 white-gray to ash-gray. Soluble yellow- 

 brown pigment. 



Glucose broth: Growth in the form of 

 sediment, white, later light yellow. No 

 aerial mycelium. Soluble citron-yellow pig- 

 ment. 



Milk: Lichenoid, light yellow growth. 

 Aerial mycelium powder}', gray-white. 

 Soluble light brown pigment. Actively pep- 

 tonized. 



Potato: Moderate, brownish yellow to 

 light brown growth. Aerial mycelium pow- 

 dery, gray-white. Soluble citron-yellow to 

 sulfur-yellow pigment. 



