768 



ORDER V. ACTINOMYCETALES 



Agar: Cream-colored growth. Thin aerial 

 mycelium. 



Sj^nthetic agar: Thin, gray, spreading 

 growth. Aerial mycelium white, becoming 

 drab gray. 



Starch agar: Thin, colorless, spreading 

 growth. Aerial mycelium gray. 



Glucose agar: Yellowish, spreading 

 growth. No aerial mycelium. 



Glucose broth: Gray ring with grayish 

 colonies in bottom of tube. 



Litmus milk: Brownish ring; coagulated; 

 peptonized in 25 to 30 days, becoming 

 faintly alkaline. 



Potato: Abundant, wrinkled, cream- 

 colored growth with greenish tinge. 



Starch is hydrolyzed. 



Brown to dark brown soluble pigment 

 formed. 



Nitrites produced from nitrates. 



Aerobic. 



Optimum temperature, 37° C. 



Antagonistic properties: Limited. 



Source: Isolated from soil. 



Habitat: Soil. 



35. Streptomyces canescens Hickey et 

 al., 1952. {Streptomijces canescus (sic) 

 Hickey, Corum, Hidy, Cohen, Nager and 

 Kropp, Antibiotics and Chemotherapy, 2, 

 1952, 472.) 



ca.nes'cens. L. part. adj. canescens be- 

 coming white, or hoary. 



Aerial mycelium: At first white, becom- 

 ing, on sporulation, gray-white to gray. 

 Conidiophores straight or curved, not form- 

 ing any spirals, richly septate. Spores 

 globose, 1.0 to 1.3 by 1.3 to 2.6 microns. 



Gelatin: Rapid liquefaction. 



Sabouraud's agar: Growth first white, 

 dull-shiny, spreading, translucent with tan 

 reverse. After 7 days' incubation, growth 

 beaded, slightly wrinkled at base of the 

 slant, grayish white; reverse tan to amber. 

 Amber pigment diffused throughout me- 

 dium. After 14 days aerial mycelium faintly 

 greenish. 



Bennett's agar: Circular colonies, effuse 

 to conve.x, edge filamentous; powdery; vary- 

 ing from gray-white to gray. Reverse brown. 

 No soluble pigment. 



Egg medium: Tan, very wrinkled surface 

 growth. No sporulation observed after 10 



days' incubation; small amount of white 

 sporulation observed in 14 days. Soluble 

 brown pigment. After 21 days, odor of hy- 

 drogen sulfide detected. Liquefaction after 

 28 days. 



Ca-malate agar: Gray to rose-gray my- 

 celium with yellow to tan reverse. Diges- 

 tion of calcium malate slight at edge of 

 colony. No soluble pigment. 



Glucose, arabinose, trehalose, xylose, su- 

 crose, maltose, galactose, dextrin, soluble 

 starch, mannitol, glycerol and salicin are 

 used as sole sources of carbon. No growth 

 observed with sorbose, melezitose, dulcitol, 

 rhamnose, sorbitol, melibiose, phenol, 

 raffinose or lactose. 



Milk: At 36° C, becomes alkaline (pH 

 8.4). Soft, rennet curd, formed after 48 

 hours, is completely peptonized in 12 days. 



Potato: Light gray, spreading, wrinkled 

 growth. Deep brown pigment diffused 

 throughout. 



Starch is actively hydrolyzed. 



Nitrites not produced from nitrates. 



C. 

 Produces 



a contaminated 



Optimum temperature, I 



Antagonistic properties 

 cosin. 



Source : Isolated from 

 fungus plate. 



Habitat: Unknown. 



36. Streptomyces finiicarius (Duch^, 

 1934) Waksman and Henrici, 1948. {Actino- 

 myces fimicarius Duche, Encyclop^die My- 

 cologique, Paris, 6, 1934, 346; Waksman and 

 Henrici, in Manual, 6th ed., 1948, 940.) 



fi.mi.ca'ri.us. L. noun fim us dung, ma- 

 nure; L. adj. cams dear, loving; M.L. adj. 

 fimicarius dung-loving. 



Gelatin: Punctiform colonies with whitish 

 aerial mycelium; reddish soluble pigment. 

 Liquefaction. 



Agar: Cream-colored growth with white 

 aerial mycelium; reverse side, yellowish. 



Asparagine agar: Cream-colored growth 

 with whitish aerial mycelium; reverse side 

 cream-colored to slight ochre. 



Synthetic agar: Yellowish masses of 

 growth with j^ellowish white aerial my- 

 celium; reverse side orange-colored; faint 

 yellowish soluble pigment. 



Asparagine solution: Vegetative filaments 

 0.5 to 0.6 micron long; branching aerial 



