770 



ORDER V. ACTINOMYCETALES 



Synthetic glycerol agar: Colorless to 

 brownish, restricted growth. Scant white 

 to dark grayish colored aerial mj^celium. 

 Soluble brown pigment. 



Egg media: Reddish brown, wrinkled 

 growth. No aerial mycelium. No soluble 

 pigment. 



Loeffler's blood serum: Elevated, 

 wrinkled, colorless to brownish growth. No 

 aerial mycelium. No soluble pigment. No 

 liquefaction. 



Blood agar: Brownish, wrinkled, poor 

 growth. No aerial mycelium. No soluble 

 pigment. No hemolysis. 



Milk: Poor surface growth. No aerial 

 mycelium. No soluble pigment. Coagulated 

 and slowly peptonized. 



Potato plug: Yellowish brown to brown- 

 ish, fine, wrinkled growth. White, powdery 

 aerial mycelium. No soluble pigment at 

 first, later reddish brown. 



Nitrites produced from nitrates. 



Antagonistic properties: Produces an 

 antiviral agent, achromoviromycin. 



Distinctive characters: This species re- 

 sembles Sfreptomyces diastaticus and Strep- 

 tomyces fimicarius. This species differs from 

 the former in spiral formation, hemolysis, 

 liquefaction of gelatin and proteolj^tic ac- 

 tion of milk. It differs from the latter in 

 the liquefaction of coagulated serum. This 

 species is characterized by the brown pig- 

 mentation on synthetic agar only. 



Source: Isolated from garden soil at 

 Suginami-ku, Tokyo. 



Habitat: Soil. 



39. Streptoniyces noursei Brown et 

 al., 1953. (A soil actinomycete, Hazen and 

 Brown, Science, 112, 1950, 423; Streptoniyces 

 sp. No. 48240, Hazen and Brown, Proc. 

 Soc. Exp. Biol, and Med., 76, 1951, 93; 

 Brown, Hazen and Mason, Science, 117, 

 1953, 609.) 



nour'se.i. M.L. gen. noun noursei of 

 Nourse; named for the owner of the farm 

 where the soil sample was obtained from 

 which this organism was isolated. 



Description prepared by Hazen and 

 Brown for use in Waksman and Lechevalier, 

 Actinomycetes and Their Antibiotics, 

 Baltimore, 1953. 



Vegetative growth: Good growth, gray 



to brown, much folded on various organic 

 and certain synthetic media. Soluble pink 

 to purple pigment frequently produced. 



Aerial mycelium: Well developed, white 

 to shell-pink. Straight, curved and spiral- 

 forming sporulating hyphae. Round to el- 

 lipsoidal spores. 



Gelatin: Rapid liquefaction. 



Synthetic agar: Scant, colorless, flat 

 growth. No aerial mj^celium. 



Glucose asparagine agar: Wrinkled, tan 

 growth with gray and white knob-like pro- 

 jections. Dark gray reverse. At 35° to 36°C., 

 scant, shell-pink aerial mycelium; limited, 

 shell-pink soluble pigment. 



Starch agar: Good growth in form of dis- 

 crete colonies with white aerial mycelium 

 in center and periphery colorless and em- 

 bedded. 



Glucose agar: Good, folded growth. 

 White aerial mycelium, turning gray. Re- 

 verse of growth brown, medium often be- 

 coming darkened throughout. Occasionally 

 pomegranate-purple soluble pigment is 

 formed. 



Blood agar: Good growth, consisting of 

 convex, lobate colonies with central perfora- 

 tion. Aerial mycelium heavy, chalky white. 

 No hemolysis, but darkening of blood. 



Honey broth: Heavy white ring on sur- 

 face; flocculent sediment. Broth clear. 



Milk: Coagulated then peptonized. 



Potato: Good, folded growth with chalky 

 white aerial mycelium. At 35° to 36° C, a 

 reddish purple pigment is formed. 



Starch is hydrolyzed. 



Poor growth on cellulose. 



Nitrites weakly produced from nitrates. 



Antagonistic properties: Produces an 

 antifungal agent, nystatin, which is active 

 against various yeast-like and filamentous 

 fungi, and an antibacterial agent, phala- 

 mycin. 



Source: Isolated from soil from Fauquier 

 County, Virginia. 



Habitat: Soil. 



40. Streptoniyces roseochromogenes 



(Jensen, 1931) Waksman and Henrici, 1948. 

 (Actinomyces roseus Krainsky, Cent. f. 

 Bakt., II Abt., 41, 1914, 662; also see Waks- 

 man and Curtis, Soil Sci., 1, 1916, 125; and 

 Waksman, Soil Sci., 8, 1919, 148; not Acti- 



