786 



ORDER V. ACTINOMYCETALES 



colored to straw-colored. No soluble pig- 

 ment. 



Glucose agar: Cream-colored to brownish 

 growth. Aerial mycelium abundant, cream- 

 colored. Faint soluble brownish pigment. 



Broth: Colorless surface film. Aerial my- 

 celium heavy, cream-colored. No soluble 

 pigment. 



Milk: Colorless to brownish surface ring. 

 No aerial mycelium. Not coagulated; pep- 

 tonized. 



Potato: Folded, brownish growth. Aerial 

 mj^celium cream-colored. Faint, soluble 

 pigment. 



Sucrose is inverted. 



Starch is actively hydrol.yzed. 



Good growth on cellulose. 



Paraffin and fats support good growth. 



Nitrites produced from nitrates. 



Odor: Strong, characteristic of soil. 



Antagonistic properties: Some strains 

 give positive effects, others are negative. 



Source : Isolated from sputum in a case of 

 chronic bronchitis. 



Habitat: Unknown. 



74. Strep tomyces roseoflavus Arai, 

 1951. (Jour. Antibiotics (Japanese), 4, 1951, 

 215; see Biol. Abst., 21, 1953, No. 1867.) 



ro.se. o.fia'vus. L. fem.n. rosa a rose; L. 

 adj . jlavus yellow; M.L. adj . roseoflavus rose- 

 yellow. 



Vegetative growth: Large, flat colonies; 

 vegetative mycelium limited to medium. 



Aerial mycelium: White to rose-colored, 

 producing numerous spirals. Spores ellip- 

 soidal to oblong, 0.8 to 1.0 by 1.0 to 1.8 

 microns. 



Gelatin: Strong liquefaction. Orange- 

 brown colonies at bottom of liquefied zone. 

 No soluble pigment. 



Agar: Much-folded, white-gray to golden 

 j^ellow growth. White to rose-colored aerial 

 mycelium, limited to center of colonies. 



Sjmthetic agar: Colorless to j-ellowish 

 growth, penetrating deep into medium. 

 Powdery, white to .yellow-rose aerial my- 

 celium. 



Starch agar: Golden yellow growth with 

 whitish aerial mycelium. 



Glucose agar: Colorless to yellowish white 

 growth with rose-colored aerial mj^celium. 



Milk: Cream-colored surface ring. Rap- 



idly coagulated and peptonized, the medium 

 becoming strongly alkaline. 



Potato: Yellow mass without aerial my- 

 celium. No soluble pigment. 



Fair growth on cellulose paper; cellulose is 

 decomposed. 



Nitrites produced from nitrates. 



Antagonistic properties : Produces a basic 

 antibiotic, flavomycin, similar to neomycin. 



Comment: Culture similar to that of 

 Streptomyces microflavus. 



Source: Isolated from garden soil from 

 Chiba and Hiroshima. 



Habitat: Soil. 



75. Streptomyces putrificus (Nicolai- 

 eva, 1915) Waksman, 1953. [Actinomyces 

 puirificus Nicolaieva, Arch. Biol. Nauk, 18, 

 1915, 240; Streptomyces putrificans (sic) 

 Waksman, in Waksman and Lechevalier, 

 Actinomj^cetes and Their Antibiotics, Bal- 

 timore, 1953, 81.) 



pu.tri'fi.cus. L. V. putrefacio to make 

 rotten; M.L. adj. putrificus making rotten. 



Vegetative growth: Colorless. 



Aerial mycelium: White. 



Agar: Grayish growth covered with white 

 aerial mycelium. No soluble pigment. 



LoefHer's serum: Yellow growth. No aerial 

 mycelium. Serum liquefied and colored 

 j'ellowish brown. 



Glucose broth: Yellow soluble pigment. 



Milk: Heavy pellicle, covered with white 

 aerial mycelium. Gradually peptonized 

 without previous coagulation. 



Potato: Folded, sulfur-j-ellow growth. 

 Aerial mycelium chalk-white. No soluble 

 pigment. 



Proteins energetically decomposed with 

 the production of bad-smelling products, 

 hydrogen sulfide and ammonia. 



Odor: Strong, putrefactive. 



Relationships to other species : Similar to 

 Actinomyces albus Krainsky but differs 

 from it in that it putrefies proteins with 

 the production of gases. 



Source : Isolated from surface water near 

 St. Petersburg. 



Habitat: Unknown. 



76. Streptomyces citreus (Krainsky, 

 1914) Waksman and Henrici, 1948. (Actino- 

 myces citreus Krainsky, Cent. f. Bakt., II 



