'92 



ORDER V. ACTINOMYCETALES 



lon.gis'si.mus. L. sup. adj. longissimus 

 longest, very long. 



Vegetative growth: Bright orange or gol- 

 den red colonies. No diffusible pigment. 



Aerial mycelium: Weakly developed; ab- 

 sent on some media. Sporophores straight, 

 short, single or branched. Spores elongated, 

 0.7 by 1.0 to 1.3 microns, cylindrical. 



Gelatin: Rapid liquefaction. 



Synthetic agar: Well developed, velvety, 

 white-yellow aerial mycelium. 



Potato: Well developed aerial mycelium 

 of characteristic color. 



Starch is actively hydrolyzed. 



Good growth on cellulose. 



Paraffin and waxes support good growth. 



Antagonistic properties: Weakly antago- 

 nistic. 



Relationships to other species: Similar to 

 Strepiomyces fradiae. 



Source: Isolated from soil. 



Habitat: Soil. 



87. Streptomyces olivaceus (Waks- 

 man, 1923) Waksman and Henrici, 1948. 

 (Actinomyces 206, Waksman, Soil Sci., 7, 

 1919, 117; Actinomyces olivaceus Waksman, 

 in Manual, 1st ed., 1923, 354; Waksman and 

 Henrici, in Manual, 6th ed., 1948, 950.) 



o.li.va'ce.us. L.noun oliva the olive; 

 M.L. adj. olivaceus olive-colored. 



Aerial mycelium: Clumps small, with 

 straight and branching hyphae. No spirals 

 on most media. Conidia spherical and 

 ellipsoidal, 0.9 to 1.1 by 0.9 to 2.0 microns. 



Gelatin stab: Liquefaction with cream- 

 colored, flaky, yellow sediment. 



Agar: White, glistening growth. 



Synthetic agar: Growth abundant, 

 spreading, developing deep into medium, 

 yellow to olive -ochre, reverse yellow to 

 almost black. Aerial mycelium mouse-gray 

 to light drab. 



Starch agar: Thin, yellowish green, 

 spreading growth. 



Glucose agar: Growth abundant, restric- 

 ted, entire, center raised. 



Glucose broth: Sulfur-yellow ring. 



Litmus milk: Faint, pinkish growth; co- 

 agulated; peptonized, becoming alkaline. 



Potato: Growth abundant, much wrin- 

 kled, elevated, gray, turning sulfur-yellow 

 on edge. 



The pigment formed is not soluble. 



Starch is hydrolyzed. 



Nitrites produced from nitrates. 



Aerobic. 



Optimum temperature, 25° C. 



Antagonistic properties: Positive. 



Source: Isolated from soil. 



Habitat: Soil. 



88. Streptomyces microflavus (Krain- 

 sky, 1914) Waksman and Henrici, 1948. 

 (Actinomyces microflavus Krainsky, Cent. f. 

 Bakt., II Abt., 41, 1914, 662; Waksman and 

 Henrici, in Manual, 6th ed., 1948, 950.) 



micro. fla'vus. Gr. adj. micrus small; 

 L. adj. flavus yellow; M. L. adj. microflaviis 

 somewhat yellow. 



Aerial mycelium: Well developed, cot- 

 tony, white. Spores spherical to rod-shaped, 

 often in pairs or chains, 2.0 by 2.0 to 5.0 

 microns. 



Gelatin colonies: Small, yellow. 



Gelatin stab: Rapid liquefaction. 



Agar: Yellow colonies with rose-yellow 

 aerial mycelium in 3 to 4 weeks. 



Ca-malate agar: Minute yellow colonies. 

 No aerial mycelium. 



Glucose agar: A rose-yellow aerial my- 

 celium develops in about 12 days. 



Starch agar: Same as on glucose agar. 



Glucose broth: Small spherical colonies in 

 depth. 



Litmus milk : Rapidly coagulated and pep- 

 tonized. 



Potato: Yellow growth. No aerial my- 

 celium. 



Diastatic action strong. 



Scant growth on cellulose. 



Nitrites produced from nitrates. 



Aerobic. 



Optimum temperature, 25° C. 



Antagonistic properties: Positive. 



Source: Isolated from soil. 



Habitat: Soil. 



89. Streptomyces cacaoi (Waksman, 

 1932) Waksman and Henrici, 1948. (Actino- 

 myces cacaoi Waksman, in Bunting, Ann. 

 Appl. Biol., 19, 1932, 515; Waksman and 

 Henrici, in Manual, 6th ed., 1948, 951.) 



ca.ca'o.i. Mexican Spanish cacao the ca- 

 cao tree; M.L. gen. noun cacaoi of cacao. 

 Aerial mycelium: Long with considerable 



