796 



ORDER V. ACTINOMYCETALES 



Gelatin stab: Liquefaction, with small 

 cream-colored masses in bottom of tube. 



Agar: Restricted, wrinkled, cream-coloi-ed 

 growth. 



Synthetic agar: Growth abundant, heavy, 

 spreading, raised, light, becoming dark, 

 almost black. Aerial mycelium white, turn- 

 ing dull gray. 



Starch agar: Abundant, brownish, glossy 

 growth. 



Glucose agar: Growth spreading, color- 

 less, wrinkled, center elevated, edge lich- 

 enoid, becoming brown. 



Glucose broth: Small, colorless colonies 

 in bottom of tube. 



Litmus milk: Cream-colored ring; coagu- 

 lated; peptonized, becoming alkaline. 



Potato: Growth abundant, moist, wrin- 

 kled, cream-colored with green tinge. 



The pigment formed is not soluble. 



Starch is hydrolyzed. 



Nitrites produced from nitrates. 



Aerobic. 



Optimum temperature, 37° C. 



Antagonistic properties: Strongly an- 

 tagonistic; some strains show only anti- 

 fungal activity; some strains produce 

 magnamycin. 



Source: Lsolated many times from the 

 deeper soil layers. 



Habitat: Common in subsoil. 



97. Streptoniyces funiosus (Krassilni- 

 kov, 1941) Waksman, 1953. (Actinomyces 

 fumosus Krassilnikov, Guide to the Actino- 

 mycetes, Izd. Akad. Nauk, U.S.S.R., 

 Moskau, 1941, 58; Waksman, in Waksman 

 and Lechevalier, Actinomycetes and Their 

 Antibiotics, Baltimore, 1953, 92.) 



fu.mo'sus. L. adj. fumosus full of smoke, 

 smoky. 



Aerial mycelium: Sporophores straight; 

 spores cylindrical, 0.7 by 1.5 to 2.0 microns, 

 later round. 



Gelatin: Liquefaction. 



Agar: Dark brown growth; aerial my- 

 celium white. Medium brown-colored. 



Synthetic agar: Dark brown growth. Pig- 

 ment not soluble. Aerial mycelium well 

 developed, cottony, dusty colored, occa- 

 sionally gray-white. 



Milk: Not coagulated; slowly liquefied 



with production of a dark brown to almost 

 black pigment. 



Potato: No growth, or only faint dark 

 gray aerial mycelium. 



Sucrose weakly inverted. 



Starch is actively hydrolyzed. 



No growth on cellulose. 



Antagonistic properties: None. 



Source: Isolated from soil. 



Habitat: Soil. 



98. Streptoniyces lipmanii (Waksman 

 and Curtis, 1916) Waksman and Henrici, 

 1948. {Actinomyces li-pmanii Waksman and 

 Curtis, Soil Sci., 7, 1916, 123; Waksman and 

 Henrici, in Manual, 6th ed., 1948, 952.) 



lip.ma'ni.i. M.L. gen. noun lipmanii of 

 Lipman; named for Prof. J. G. Lipman of 

 the New Jersey Agr. Exp. Station. 



Aerial mycelium: Straight, branching 

 mycelium and hyphae. Conidia ellipsoidal, 

 0.8 to 1.1 by 1.0 to 1.5 microns. 



Gelatin stab: Liquefaction, with cream- 

 colored, flaky sediment. 



Agar: Yellow, glossy, radiately wrinkled 

 growth. 



Synthetic agar: Growth abundant, raised, 

 colorless, becoming light brown and wrin- 

 kled. Aerial mycelium white, turning gray. 



Starch agar: Transparent growth, be- 

 coming dark with age. 



Glucose agar: Light yellow, irregular, 

 spreading growth. 



Glucose broth: White ring, with abun- 

 dant, colorless flaky sediment. 



Litmus milk: Cream-colored ring; coagu- 

 lated; peptonization with alkaline reaction. 



Potato: Abundant, cream-colored, wrin- 

 kled growth. 



The pigment formed is not soluble. 



Starch is hydrolyzed. 



Nitrites produced from nitrates. 



Aerobic. 



Optimum temperature, 25° C. 



Antagonistic properties: Good, though 

 some strains show no activity. 



Source : Isolated many times from a vari- 

 ety of soils. 



Habitat: Common in soil. 



99. Streptoniyces hygroscopicus (Jen- 

 sen, 1931) Waksman and Henrici, 1948. 

 {Actinomyces hygroscopicus Jensen, Proc. 



