802 



ORDER V. ACTINOMYCETALES 



Starch agar: Same as on Ca-malate agar. 



Glucose broth: Colorless, spherical 

 colonies. 



Litmus milk: Peptonized. 



Sucrose is inverted. 



Starch is actively hydrolyzed. 



Cellulose is decomposed. 



Nitrites produced from nitrates. 



Aerobic. 



Optimum temperature, 25° C. 



Antagonistic properties: Strong effect 

 upon various bacteria; some strains show no 

 activity. 



Source: Isolated from soil. 



Habitat: Soil. 



111. Streptomyces acidophilus (Jen- 

 sen, 1928) Waksman and Henrici, 1948. 

 (Actinomyces acidophilus Jensen, Soil Sci., 

 25, 1928, 226; Waksman and Henrici, in 

 Manual, 6th ed., 1948, 956.) 



a.ci.do'phi.lus. L. adj. acidus sour; M.L. 

 noun acidum an acid; Gr. adj. philus loving; 

 M.L. adj. acidophilus acid-loving. 



Vegetative growth: Mycelium profusely 

 branched, hyphae 0.6 to 0.8 micron in 

 diameter with homogeneous protoplasm and 

 no visible septa. 



Aerial mycelium: Hyphae 1.0 to 1.2 mi- 

 crons in diameter, somewhat branched, 

 forming either very few or very numerous 

 sinistrorse spirals. Ellipsoidal conidia 1.0 

 to 1.2 by 1.2 to 1.5 microns. 



Gelatin: After 10 days, growth very 

 scant, thin, colorless, semi-transparent. 

 Slow liquefaction. 



Agar: No growth. 



Synthetic agar: No growth. 



Glucose agar: Good growth at 25° C. 

 Substratum mycelium raised, somewhat 

 wrinkled, colorless in young cultures. 

 Aerial mycelium thin, white at first, later 

 gray or yellowish brown. 



Starch agar: Good growth at 25° C. 

 Substratum mycelium flat, smooth, color- 

 less. Aerial mycelium abundant, smooth, 

 white. 



Broth: No growth. 



Milk: No growth. 



Potato: Growth good, raised, folded. 

 No discoloration. 



Sucrose not inverted. 



Diastatic. 



Weakly proteolytic. 



Nitrites not produced from nitrates 

 except a trace in two strains. 



Antagonistic properties: Strongly posi- 

 tive. 



Distinctive character: Ability to live in 

 acid media only. 



Source: Four strains were isolated from 

 three acid humus soils. 



Habitat: Found in acid humus soils. 



112. Streptomyces rubesceiis (Jarach, 

 1931) Waksman and Henrici, 1948. (Strepto- 

 thrix rubescens Jarach, Boll. Sez. Ital. Soc. 

 Intern. Microb., 3, 1931, 43; Waksman and 

 Henrici, in Manual, 6th ed., 1948, 956.) 



ru.bes'cens. L. part. adj. rubescens be- 

 coming red. 



Original description supplemented by 

 material taken from Umezawa, Tazaki and 

 Fukuyama (Jour. Antibiotics (Japanese), 

 5, 1952,469). 



Aerial mycelium: Powdery, white. Micro- 

 scopically, aerial mycelium short, curved, 

 well branched, bearing spherical or ellipsoi- 

 dal conidia in chains. No spirals. 



Gelatin: Coral -pink surface growth. No 

 liquefaction and no pigmentation of me- 

 dium. 



Agar: Submerged mycelium at first white, 

 changing to salmon -pink after about 10 

 days' incubation. No soluble pigment. Re- 

 verse of growth changes to salmon-pink. 



Synthetic agar: Same as on plain agar. 



Glucose agar: Large number of small 

 round colonies raised in the center and 

 growing together as well as deep into the 

 medium; whitish opalescent color. 



Milk agar medium: Rose-coral-colored, 

 thin growth with edge entire. 



Loeffler's coagulated serum: Same as on 

 plain agar. No hydrolysis. 



Blood agar: After 10 daj^s' incubation, 

 mycelium becomes salmon-pink and bears 

 powdery white aerial mycelium. No soluble 

 pigment. No hemolysis. 



Broth: Minute flakes, the liquid later be- 

 coming reddish colored. 



Glucose broth: Surface growth with white 

 powdery aerial mycelium becomes salmon- 

 pink or coral-pink. The liquid later becomes 

 coral-red. 



Milk; Coral -pink growth; aerial mycelium 



