ORDER V. ACTINOMYCETALES 



with abundant, white to green to olive-buff 

 aerial mj'celium. Soluble, brownish to black 

 pigment. 



Nitrites produced from nitrates. 



Starch is hydrolyzed. 



Grows well at 37.5° C. 



Comment: According to Krassilnikov, 

 Streptomyces flavus, Streptomyces margina- 

 tus, Streptomyces praefecundus , Streptomyces 

 tenuis and Streptomyces loidensis represent 

 merely strains of this species. 



Source: Isolated from a small, unruptured 

 potato scab. 



Habitat: Found in potatoes so far as 

 known. 



125. Streptomyces praefecundus (Mil- 

 lard and Burr, 1926) Waksman, 1953. (Ac- 

 tinomyces praefecundus Millard and Burr, 

 Ann. Appl. Biol., 13, 1926, 601; Waksman, 

 in Waksman and Lechevalier, Actinomy- 

 cetes and Their Antibiotics, Baltimore, 

 1953, 108.) 



prae.fe.cun'dus. L. adj. praefecundus very 

 fruitful. 



Aerial mycelium: Spores ellipsoidal, 0.8 

 by 0.85 micron. 



Gelatin: Good surface growth covered 

 with aerial mycelium. Light pink to dark 

 golden brown soluble pigment. Rapid lique- 

 faction. 



Synthetic sucrose agar: Good, cream- 

 colored growth covered with abundant, 

 cottony, olive-buff aerial mycelium. Soluble 

 cream-colored pigment. 



Synthetic glucose agar: Pale olive-gray 

 growth covered with abundant, smooth, 

 olive-buff aerial mycelium. Soluble, light 

 golden to buff pigment. 



Potato agar: Lichenoid, gray growth. 

 Aerial mycelium smooth, white to yellowish. 

 Soluble golden brown pigment. 



Glycerol synthetic solution: Heavy sur- 

 face growth covered with white to olive-buff 

 aerial mycelium. Soluble pigment none or 

 very slightly green. 



Glucose broth: Masses throughout me- 

 dium flaky, whitish. Aerial mycelium abun- 

 dant, pale olive-buff. Soluble, light golden 

 brown pigment. 



Milk: Good surface growth covered with 

 scant, white aerial mycelium. Coagulation 

 followed by peptonization. 



Potato: Good, wrinkled growth covered 

 with abundant, white to yellowish to olive - 

 buff aerial mycelium. Soluble, gray to 

 brown pigment. 



Starch is hydrolyzed. 



Tyrosinase reaction: Negative. 



Nitrites produced from nitrates. 



Grows well at 37.5° C. 



Source: Isolated from a large, ruptured 

 potato scab. 



Habitat: Found in potatoes and soil so 

 far as known. 



126. Streptomyces viridogenes Waks- 

 man, 1953. (Actinomyces viridis Millard and 

 Burr, Ann. Appl. Biol., IS, 1926, 601; not 

 Actinomyces viridis Sanfelice, Cent. f. Bakt., 

 I Abt., Grig., 36, 1904, 355; Waksman, in 

 Waksman and Lechevalier, Actinomycetes 

 and Their Antibiotics, Baltimore, 1953, 109.) 



vi.ri.do'ge.nes. L. adj. viridis green; 

 Gr. v.suffix -genes producing; M.L. adj. viri- 

 dogenes green-producing. 



Aerial mycelium: Long, straight fila- 

 ments. Spores spherical, 0.9 micron. 



Gelatin: Grayish growth with scant, white 

 to gray, aerial mycelium. Soluble light 

 golden brown pigment. Rapid liquefaction. 



Agar: Lichenoid, cream-colored growth. 

 No aerial mycelium. No soluble pigment. 



Sj^nthetic sucrose agar: Abundant growth 

 covered with olive-grayish aerial mycelium. 

 Soluble greenish yellow to blackish green 

 pigment . 



Synthetic glucose agar: Smooth, raised, 

 olive-buff growth. Abundant aerial myce- 

 lium, light gray to deep mouse-gray. Solu- 

 ble yellowish to greenish yellow pigment. 



Glucose agar: Gray to black growth. 

 Aerial mj'celium gray. Soluble dark brown 

 pigment. 



Starch agar: Gray to brown growth. 

 Aerial mycelium thin, white. 



Broth: Thin surface growth and flaky, 

 cream-colored growth in bottom. No solu- 

 ble pigment. 



Glycerol synthetic solution: Flaky, white 

 to vinaceous growth in medium and on 

 surface. Aerial mycelium gray. 



Glucose broth: Growth on surface and at 

 base good, flakj'. Aerial mycelium gray to 

 mouse-gray. Soluble vinaceous to cinnamon- 

 colored pigment. 



