812 



ORDER V. ACTINOMYCETALES 



Synthetic glucose agar: Thin, flat, almost 

 black growth with greenish tinge. 



Potato agar: Vinaceous, tawny colored 

 growth producing a vinaceous tawny soluble 

 pigment. 



Glycerol synthetic solution: Poor, flaky 

 growth at bottom. 



Glucose broth: Numerous minute, whitish 

 colonies at bottom of container. 



Milk: Very slight growth. Not coagulated; 

 not peptonized. 



Potato: Restricted, raised, pinkish 

 growth. Aerial mycelium scant, white. 

 Soluble, gray to brown pigment. 



Starch is hydrolyzed. 



Tyrosinase reaction: Negative. 



Nitrites not produced from nitrates. 



Grows well under anaerobic conditions. 



Grows well at 37.5° C. 



Source: Isolated from sour soil. 



Habitat: Soil. 



134. Streptoniyces coroniformis (Mil- 

 lard and Burr, 1926) Waksman, 1953. (.4c- 

 tinomyces coroniformis Millard and Burr, 

 Ann. Appl. Biol., 13, 1926, 601; Waksman, 

 in Waksman and Lechevalier, Actinomy- 

 cetes and Their Antibiotics, Baltimore, 

 1953, 114.) 



co.ro.ni.for'mis. L. fem.n. corona crown; 

 L. ievti.n. forma form; M.L. adj. coroniformis 

 crown-shaped. 



Aerial mycelium: Simple branching. 

 Spores ellipsoidal, 0.6 by 0.8 micron. 



Gelatin: Fair growth. Weak or no lique- 

 faction. 



Synthetic sucrose agar: Gray to greenish 

 discrete colonies partially coalescing. White 

 aerial mycelium covering edges of growth. 



Synthetic glucose agar: Grayish discrete 

 colonies growing into medium weakly pro- 

 duced. No aerial mycelium. 



Potato agar: Wrinkled, grayish growth. 

 No aerial mycelium. 



Glycerol synthetic solution: Very poor 

 growth in form of small flakes at bottom. 



Glucose broth: Fair growth at bottom. 



Milk: A few colonies on surface. Not co- 

 agulated; weakly peptonized. 



Potato: Raised, grayish growth. Aerial 

 mycelium white. Plug pigmented brownish 

 around and under growth. 



Starch may be weakly hydrolyzed. 



Tyrosinase reaction: Negative. 

 Nitrites weakly produced from nitrates. 

 Fair growth at 37.5° C. 

 Source: Isolated from peat soil. 

 Habitat: Soil. 



135. Streptoniyces spiralis (Millard and 

 Burr, 1926) Waksman, 1953. [Actinomyces 

 spiralis Millard and Burr, Ann. Appl. Biol., 

 13, 1926, 601; Waksman, in Waksman and 

 Lechevalier, Actinomycetes and Their 

 Antibiotics, Baltimore, 1953, 114.) 



spi.ra'lis. Gr. fem.n. spira a spiral; M.L. 

 adj. spiralis like a spiral. 



Vegetative growth: Lichenoid, smooth, 

 yellowish golden growth. No soluble pig- 

 ment . 



Aerial mycelium: Sporophores straight or 

 spiral-forming. Spores cylindrical, 0.9 by 

 1.0 to 1.7 microns. 



Gelatin: Limited, gray growth. Aerial 

 mycelium scant, white. Rapid liquefaction. 



Synthetic sucrose agar: Rough or granu- 

 lar growth. Aerial mycelium vinaceous buff 

 to dark grayish olive. Soluble, pale vina- 

 ceous-f awn-colored pigment. 



Synthetic glucose agar: Fairly rough, 

 pale gray growth. Aerial mycelium scant, 

 white. Soluble, j'ellowish to pink pigment. 



Potato agar: Granular, gray growth. 

 Aerial mycelium abundant, white to olive- 

 buff. Soluble, light golden brown pigment. 



Glycerol synthetic solution: No growth. 



Glucose broth: Minute compact colonies 

 at bottom and on surface. Aerial mycelium 

 scant, white. 



Milk: Good surface growth covered wdth 

 abundant, white aerial mycelium. Coagu- 

 lated, rapidly peptonized. 



Potato: Poor, wrinkled, grayish vina- 

 ceous growth. Aerial mycelium w^hite to 

 grayish vinaceous. Plug colored brown 

 around and below growth. 



Starch is not hydrolyzed. 



Tyrosinase reaction: Negative. 



Nitrites not produced from nitrates. 



Does not grow at 37.5° C. 



Source: Isolated from a heap of decaying 

 grass. 



Habitat: Found in grass compost so far 

 as known. 



136. Streptoniyces sampsonii (Millard 



