814 



ORDER V. ACTINOMYCETALES 



name of the sweet potato; M.L. gen. noun 

 ipomoeae of Ipoinoea. 



Conidia on glucose-casein agar: Ellipsoi- 

 dal to elliptical, 0.9 to 1.3 by 1.3 to 1.8 mi- 

 crons. 



Gelatin: After 25 days at 20° C, scant 

 growth, no aerial mycelium; no soluble 

 pigment; liquefaction. 



Agar: Moderate growth in the form of 

 small, shiny, crinkled colonies both on the 

 surface and imbedded in the medium, sil- 

 ver-colored. 



Synthetic agar: Abundant growth, mostly 

 on surface of medium, moderately wrinkled, 

 olive-yellow. 



Starch agar: Growth moderate, smooth, 

 deep in medium, ivory-colored. Aerial 

 mycelium white with patches of bluish 

 green. No soluble pigment. Complete hy- 

 drolysis after 12 days. 



Milk: Growth in form of ring; hydroly- 

 sis, without visible coagulation. 



Potato: Growth moderate, light brown, 

 shiny, wrinkled. No aerial mycelium. No 

 soluble pigment. 



Starch is hydrolyzed. 



No growth on cellulose. 



Nitrites are produced from nitrates. 



Antagonistic properties: Positive. 



Source: Isolated from diseased sweet- 

 potato (Ipomoea sp.) tubers and small 

 rootlets from several localities in Louisiana. 



Habitat: Found in sweet potatoes so far 

 as known. 



139. Streptoniyces poolensis (Tauben- 

 haus, 1918) Waksman and Henrici, 1948. 

 {Actinomyces poolensis Taubenhaus, Jour. 

 Agr. Res., 13, 1918, 446; Waksman and Hen- 

 rici, in Manual, 6th ed., 1948, 949.) 



poo.len'sis. M.L. adj. poolensis pertaining 

 to Poole; named for Prof. R. F. Poole, a 

 plant pathologist. 



Description taken from Waksman (Soil 

 Sci., 8, 1919, 140). 



Aerial mycelium: Fine, branching; spirals 

 usually not seen. Conidia ellipsoidal. 



Gelatin stab: Liquefaction, with small, 

 brownish flakes in fluid. 



Agar: Yellowish, translucent growth. 



Synthetic agar: Thin, colorless, spread- 

 ing growth. Aerial mycelium white to gray. 



Starch agar: Restricted, cream-colored 

 growth. 



Glucose agar: Growth abundant, light 

 brown, glossy, raised center, entire. 



Glucose broth: Thin, brownish ring. 



Litmus milk: Brownish ring; coagulated; 

 peptonized, with strongly alkaline reaction. 



Potato: Thin, reddish brown; medium 

 becomes purplish. 



Faint trace of soluble brown pigment. 



Starch not hydrolyzed. 



Nitrites produced from nitrates. 



Aerobic. 



Optimum temperature, 37° C. 



Antagonistic properties: Positive. 



Source: Isolated from a sweet potato 

 "pox." 



Habitat: Unknown. 



140. Streptoniyces tumuli (Millard and 

 Beeley, 1927) Waksman, 1953. {Actinomyces 

 tumuli Millard and Beeley, Ann. Appl. 

 Biol., 14, 1927, 296; Waksman, in Waksman 

 and Lechevalier, Actinomycetes and Their 

 Antibiotics, Baltimore, 1953, 117.) 



tu'mu.li. L. mas.n. tumulus a mound; L. 

 gen. noun tumuli of a mound. 



Gelatin: Beaded growth. No aerial my- 

 celium. No soluble pigment. Rapid lique- 

 faction. 



Agar: Good, lustrous, slimy, gray growth. 

 No aerial mycelium. No soluble pigment. 



Synthetic agar: Gray growth penetrating 

 into the medium, later becoming darkly 

 opaque. Aerial mycelium arises on center 

 of growth, at first white, later becoming 

 pale gray. Surface of growth covered with 

 colorless drops leaving small, black craters. 

 No soluble pigment. 



Glucose agar: Wrinkled, pale gray growth. 

 White aerial mycelium arising in concentric 

 rings around a dark bare center. Soluble, 

 olive-colored pigment. 



Broth: Large, spherical, white colonies. 

 No soluble pigment. 



Milk: Good growth. No aerial mycelium. 

 Coagulated; slightly peptonized. 



Potato: Heavy, slimy, black growth. No 

 aerial mycelium. Plug becoming grayish 

 brown. 



Starch is hydrolyzed. 



