816 



ORDER V. ACTINOMYCETALES 



Glucose-peptone medium: Numerous col- 

 onies covering surface. Soluble brownish 

 pigment weakly produced. 



Milk: Slow growth. Aerial mycelium 

 white. No soluble pigment. At 25° C, not 

 coagulated; at 37° C, coagulated after 20 

 days. Not peptonized. No change in reac- 

 tion. 



Nitrites produced from nitrates. 



Proteolysis: No action on coagulated 

 serum. 



Pathogenicity: Pathogenic for guinea 

 pigs and rabbits. Not pathogenic for dogs 

 after laboratory growth. 



Source : Isolated from cases of dog septi- 

 cemia (thoracic, abdominal and brain le- 

 sions). 



Habitat: Found in dogs, guinea pigs and 

 rabbits so far as known. 



143. Streptomyces upcottii (Erikson, 

 1935) Waksman and Henrici, 1948. (A new 

 pathogenic form of Streptothrix, Gibson, 

 Jour. Path, and Bact., 23, 1920, 357; Actino- 

 myces upcottii Erikson, Med. Res. Council 

 Spec. Rept. Ser. 203, 1935, 36; Waksman 

 and Henrici, in Manual, 6th ed., 1948, 961.) 



up.cot'ti.i. M.L. gen. noun upcottii of 

 Upcott; named for Dr. Harold Upcott, the 

 surgeon who first secured this organism. 



Description taken from Erikson (op. cit., 

 1935, 22). 



Vegetative growth: Filaments charac- 

 teristically long, straight, much interwoven 

 and ramified; typical unicellular mycelium, 

 usually forming medium to large heavy 

 cartilaginous colonies. 



Aerial mycelium: A very slight transient 

 aerial mycelium appeared on one agar slope, 

 but this has not been repeated on any slide 

 microculture on any medium. Slightly acid- 

 fast. 



Gelatin: Abundant flocculent growth 

 along streak, round cream-colored colonies 

 on surface. Partly liquefied in 14 days; 

 complete liquefaction in 2 months. 



Agar: Smooth, shining, round, cream- 

 colored colonies, margin submerged, scant 

 white aerial mycelium in one week; colonies 

 large (up to 10 mm in diameter), centers 

 elevated, greenish tinge, very sparse aerial 

 mycelium in two weeks ; the aerial mycelium 



disappears and large radial grooves appear 

 in most colonies in 3 weeks. 



Glucose agar: Smooth, round, cream- 

 colored colonies, margin depressed, centers 

 elevated, hollow on reverse side; later a 

 coherent membranous growth, piled up, 

 yellowish. 



Glycerol agar: Small, round, cream- 

 colored, glistening colonies, heavy texture, 

 margins submerged; later, colonies umbili- 

 cated, tending to be piled up; after 6 weeks, 

 growth very much convoluted and raised, 

 broad submerged margin, slightly reddish 

 medium. 



Coon's agar: Small, radiating, white 

 colonies, growth mostly submerged. 



Ca-agar: Small, colorless membranous 

 growth with undulating margin; later, cen- 

 trally depressed into medium. 



Potato agar: Poor growth, small, color- 

 less blister colonies, medium slightly dis- 

 colored. 



Dorset's egg medium: Round, flat, color- 

 less, scale-like colonies, some marked by 

 concentric rings and slightly hollowed in 

 center; growth becomes yellow-brown. 



Serum agar: Large colonies (3 to 4 mm 

 in diameter), colorless, granular, centrally 

 elevated, depressed at margin, resembling 

 limpets. 



Blood agar: Large drab heavily textured 

 colonies; no aerial mycelium; no hemolysis. 



Broth: Large coherent mass composed of 

 fluffy colonies. 



Synthetic sucrose solution: Fair growth, 

 minute white colonies. 



Carrot plug: Colorless, spreading, moist, 

 wrinkled growth in six weeks; later a dull 

 greenish brown, moist, very much wrinkled 

 and depressed skin. 



Source : Isolated from the spleen in a case 

 of acholuric jaundice. 



Habitat: Unknown. 



144. Streptomyces hortonensis (Erik- 

 son, 1935) Waksman and Henrici, 1948. 

 {Actinomyces horton (sic) Erikson, Med. Res. 

 Council Spec. Rept. Ser. 203, 1935, 36; 

 Waksman and Henrici, in Manual, 6th ed., 

 1948, 962.) 



hor.to.nen'sis. M.L. adj. hortonensis per- 



