818 



ORDER V. ACTINOMYCETALES 



Serum agar: Wrinkled, glistening, cream- 

 colored, membranous growth. 



Inspissated serum: Colorless smeary 

 growth, reverse becoming transparent, 

 starting to liquefy at base; completely 

 liquefied and brown in 12 days. 



Broth: Suspended and sedimented color- 

 less flocculi, some small round colonies. 



Synthetic sucrose solution: Abundant 

 white colonies in coherent mass near bot- 

 tom of tube; large shell -shaped masses. 



Synthetic glycerol solution: At first, a 

 few round white colonies in suspension; 

 later, large branched feathery mass at 

 bottom. 



Milk: Coagulated; later peptonized. 



Litmus milk: Medium deep blue, be- 

 coming hydrolyzed to clear purple. 



Potato plug: Colorless moist membranous 

 growth with scant white aerial mycelium at 

 top of plug. 



Starch is hydrolyzed. 



Tyrosine agar: Reaction negative. 



Source: Isolated from human spleen in a 

 case of splenic anemia. 



Habitat: Unknown. 



146. Streptomyces kimberi (Erikson, 

 1935) Waksman and Henrici, 1948. (Actino- 

 myces kimberi Erikson, Med. Res. Council 

 Spec. Rept. Ser. 203, 1935, 36; Waksman 

 and Henrici, in Manual, 6th ed., 1948, 964.) 



kim'be.ri. M.L. gen. noun kimberi of 

 Kimber; presumably named for the surgeon 

 who first secured this organism. 



Vegetative growth: Mycelium of long, 

 straight, profusely branching filaments 

 forming circumscribed colonies on all media. 



Aerial mycelium: Abundant production 

 of short, straight and branched aerial my- 

 celium; small round conidia. Non-acid-fast. 



Gelatin: Liquefaction. Smooth shining 

 colonies becoming powdery white with 

 aerial mycelium, floating on liquefied me- 

 dium. No pigmentation. 



Agar: Smooth round moist cream-colored 

 colonies, 1 mm in diameter; after 17 days, 

 white powdery aerial mycelium. 



Glucose agar: Discrete cream-colored 

 colonies becoming confluent, white aerial 

 mycelium. 



Glycerol agar: Moist cream-colored colo- 



nies becoming confluent, white aerial my- 

 celium. 



Potato agar: Extensive growth covered 

 by white powdery aerial mycelium; large 

 colorless exuded droplets. 



Wort agar: Heavy brownish lichenoid 

 colony; after 30 days, a white aerial my- 

 celium. 



Ca-agar: Dull cream-colored scaly 

 growth, covered by chalky white aerial 

 mycelium. 



Coon's agar: Extensive growth, white 

 aerial mycelium in annular arrangement. 



Synthetic agar: Small colonies covered 

 with white aerial mycelium. 



Blood agar: Many large colonies, cream- 

 colored, tough, smooth, glistening, with 

 margin depressed; no hemolysis. 



Serum agar: Moist, cream-colored honey- 

 combed skin, scant white aerial mycelium. 



Dorset's egg medium: Closely adherent 

 scale-like colonies, centrally elevated, with 

 white aerial mycelium. 



Inspissated serum: Rapid spreading 

 growth, discrete round colonies at margin, 

 completely covered with white aerial my- 

 celium, colorless transpired drops; slight 

 softening at base. 



Broth: Small round colonies in sediment 

 in 2 days; supernatant colonies with white 

 aerial mycelium and large hollow flakes in 

 sediment in 15 days; occasional reddish 

 brown coloration. 



Synthetic sucrose solution: Round white 

 colonies at bottom; later small stellate 

 colonies in suspension and a few superna- 

 tant with white aerial mycelium. 



Synthetic glycerol solution : Round white 

 colonies at bottom; later coherent mulberry- 

 like mass composed of fluffy round portions ; 

 after 15 days, irregular wispy flocculi and 

 large coherent mass. 



Milk: Coagulation; no peptonization; 

 initial pinkish brown ring descends until 

 medium is dark brown throughout (2 

 months). 



Litmus milk: Blue coloration, hydrolyzed 

 to clear purple in 2 months. 



Starch not hydrolyzed. 



Tyrosine agar: Reaction negative. 



Source: Isolated from a blood culture of 

 a woman with acholuric jaundice. 



