820 



ORDER V. ACTINOMYCETALES 



ynyces panja (sic) Erikson, Med. Res. Coun- 

 cil Spec. Kept. Ser. 203, 1935, 36; Waksman 

 and Henrici, in Manual, 6tli ed., 1948, 966.) 



pan'jae. M.L. gen. noun panjae of Panja; 

 named for Dr. Panja, who first secured this 

 organism. 



Vegetative growth: Unicellular mycelium 

 with slender, branching filaments; very 

 small, round colonies. 



Aerial mycelium: No aerial mycelium 

 visible on any medium, but occasional 

 isolated aerial branches. Non-acid-fast. 



Gelatin: Complete liquefaction in 4 days. 



Agar: Colorless irregularly piled up con- 

 voluted growth; after 1 month, easily de- 

 tachable, brownish. 



Glucose agar: Small colorless coiled mass 

 in 1 week; heaped up green growth in 2 

 weeks . 



Glycerol agar: Poor growth, scant color- 

 less patch. 



Ca-agar: Colorless to pink spreading 

 growth with minute discrete colonies at 

 margin; after 2 weeks, bright red mass, 

 buckled and shining, colorless submerged 

 margin. 



Coon's agar: Small submerged colorless 

 growth. 



Potato agar: Small elevated convoluted 

 colorless masses with purple tinge in center. 



Dorset's egg medium: Small round tough 

 colorless colonies, margin well embedded; 

 after 3 weeks, colonies elevated, warted, 

 darkened, medium discolored and broken; 

 slight degree of liquefaction, medium dark 

 brown. 



Serum agar: Colorless, glistening, piled 

 up, convoluted mass. 



Inspissated serum: Small round blister 

 colonies and irregularly convoluted patches 

 deeply sunk in pitted medium; after 2 

 weeks, medium transparent, slight degree 

 of liquefaction. 



Broth: Flakes and minute colorless 

 colonies. 



Glucose broth: Poor growth, scant flakes, 

 pinkish. 



Synthetic sucrose solution: Pinkish floc- 

 culi; after 3 weeks, moderate growth, mi- 

 nute colorless colonies. 



Milk: Coagulation; pale green surface 

 growth; mostly digested in 2 weeks. 



Litmus milk: Soft coagulum, color un- 



changed; after 2 months, mostly digested, 

 residue coagulum light purple. 



Source: Isolated from an ulcer of the 

 abdominal wall from a patient in Calcutta. 



Habitat: Unknown. 



149. Streptoiiiyces willniorei (Erikson, 

 1935) Waksman and Henrici, 1948. (Actino- 

 viyces willmorei Erikson, Med. Res. Council 

 Spec. Rept. Ser. 203, 1935, 36; Waksman 

 and Henrici, in Manual, 6th ed., 1948, 966.) 



will.mo're.i. M.L. gen. noun willmorei of 

 Willmore; named for the surgeon who first 

 secured this organism. 



Vegetative growth: Germination usual, 

 but growing unicellular mycelium fre- 

 quently branches at very short intervals, 

 presenting peculiar clubbed and budding 

 forms with occasional separate round swol- 

 len cells which may represent the cystites 

 of other writers. The filaments are charac- 

 teristically long, homogeneous, and much 

 interwoven. 



Aerial mycelium: Profuse in most media, 

 with a marked tendency to produce loose 

 spirals (water and synthetic glycerol agar) 

 with chains of ellipsoidal conidia. Thick 

 aerial clusters may also be formed. 



Gelatin: Minute colorless colonies; lique- 

 faction. 



Agar: Heavy folded colorless lichenoid 

 growth, rounded elevations covered with 

 white aerial mycelium; later, submerged 

 margin, round confluent growth, aerial 

 mycelium marked in concentric zones. 



Glucose agar: Colorless wrinkled con- 

 fluent growth with smooth entire margin, 

 large discrete colonies like flat rosettes; 

 after 4 months, scant white aerial mycelium. 



Glycerol agar: Round smooth cream- 

 colored colonies, heavy texture, margin 

 submerged, stiff sparse aerial spikes; after 

 3 weeks, colonies large (up to 10 mm in di- 

 ameter) . 



Ca-agar: Spreading colorless growth, 

 pitting medium, submerged undulating 

 margin; very scant white aerial mycelium. 



Coon's agar: Opaque white growth ex- 

 tending irregularly (up to 3 mm) into me- 

 dium, margin smooth and submerged, 

 center raised, greenish tinge covered with 

 white aerial mycelium; after 3 weeks, mar- 



