FAMILY IV. ACTINOPLANACEAE 



825 



heat; Gr. adj. philus loving; M.L. adj. thal- 

 pophilus warmth-loving. 



Vegetative growth: Grows well on organic 

 and synthetic media, except on potato, at 

 50° C. Growth is colorless with yellow pig- 

 mentation in edges; color changes to orange 

 with age of culture. Soluble, wine-colored 

 to light rose pigment produced in yeast- 

 glucose agar. 



Aerial mycelium: Well developed, white, 

 powdery, with tendency to form "fairy 

 rings" on some media. Sporulating hyphae 

 very short, 0.6 to 1.0 micron in length. 

 Spherical conidia, 0.8 to 1.5 microns in di- 

 ameter, are produced singly on the short 

 sporophores or are entirely sessile. 



Gelatin: Liquefaction. 



Synthetic sucrose agar: Abundant, color- 

 less growth. Powdery white to light gray 

 aerial mycelium. 



Glucose-asparagine agar: Colorless 

 growth with reverse white to yellow to light 

 brown. White, powdery aerial mycelium. 

 Considerable development of "fairy rings" 

 in aerial mycelium. 



Glycerol agar: Cream-colored vegetative 

 growth; white aerial mycelium. 



Yeast-glucose agar: Good, colorless 

 growth, pigmented yellow at edges, color 

 turning orange in older cultures. Lichenoid, 

 white aerial mycelium. Soluble, wine to 

 rose pigment. 



Potato agar: Limited vegetative growth, 

 penetrating deep into medium. Limited, 

 powdery, white aerial mycelium. 



Milk: Coagulated with limited peptoniza- 

 tion. 



Starch is rapidly hydrolyzed. 



Nitrites weakly produced from nitrates. 



No growth at 28° C; good growth in 4 



days at 37° C; excellent growth in 2 daj^s 

 at 50° C. 



Source: Isolated from soil and high-tem- 

 perature composts. 



Habitat: Presumably widely distributed 

 in soil. 



3. Thermoactinomyces monosporus 



(Lehmann and Schiitze, 1908) Waksman, 

 1953. (Actinomyces monosporus Lehmann 

 and Schiitze, in Schiitze, Arch. f. Hyg., 67, 

 1908, 50; Thermoactinomyces monospora 

 (sic) Waksman, in Waksman and Lecheva- 

 lier, Actinomycetes and Their Antibiotics, 

 Baltimore, 1953, 130.) 



mo.no'spo.rus. Gr. adj. monus single; Gr. 

 fem.n. spora a seed; M.L. fem.n. spora a 

 spore; M.L. adj. monosporus single-spored. 



Vegetative growth: Grows well in various 

 media. Growth yellowish, compact, smooth 

 or lichenoid. Hyphae about 1.0 micron in 

 diameter. 



Aerial mj'celium: Well developed, cover- 

 ing the whole growth, grayish green. Good 

 sporulation on hay infusion-peptone agar; 

 somewhat less on glycerol-peptone and lac- 

 tose-peptone agars; none on peptone-glu- 

 cose agar. Ellipsoidal spores, 1.0 to 1.4 by 

 1.5 to 1.8 microns; produced singly on sim- 

 ple, short sporophores. 



Gelatin: Liquefaction. 



Blood serum: Good, smooth growth. Se- 

 rum liquefied. 



Milk: Not coagulated. 



Potato: No growth. 



Optimum temperature, between 37° and 

 55° C; grows poorly at 27° and not at all 

 at 60° C. 



Source: Isolated from self-heated hay. 



Habitat: Presumably soil. 



FAMILY IV. ACTINOPLANACEAE COUCH, 1955.* 



(Actinosporangiaceae Couch, Jour. Elisha Mitchell Scientific Soc, 71, 

 1955, 149; Couch, ibid., 269.) 



Ac.ti.no.pla.na'ce.ae. M.L. noun Actinoplanes type genus of the family; -aceae ending 

 to denote a family; M.L. fem.pl.n. Actinoplanaceae the Actinoplanes family. 



* Prepared by Prof. John N. Couch, Department of Botany, University of North Caro- 

 lina, Chapel Hill, North Carolina, January, 1955. The investigational work was supported 

 by a grant from the National Science Foundation. The author gratefully acknowledges the 

 assistance of Dr. E. K. Goldie-Smith. 



