FAMILY II. CHLAMYDIACEAE 



967 



formis ovis Zhdanov, Opredelitel Virusov 

 Celovska i Zivotmych, Izd. Akad. Med. 

 Nauk, U.S.S.R., Moskau, 1953, 188.) 



o'vis L. noun ovis a sheep. 



Elementary bodies are coccoid bodies re- 

 sembling those of Miyagawanella lympho- 

 granulomatosis. Often occur in clusters. 

 Found in cell cytoplasm and free. Stain red 

 with Ziehl-Neelsen's stain. Also stain with 

 Giemsa's, with Castafieda's and with Mac- 

 chiavello's stains. 



Filterability: Passes through a 0.4 mi- 

 cron gradacol membrane. 



Cultivation: Grows in the yolk sac of the 

 chicken embryo. 



Viable after storage at —20° or —70° C. 



Pathogenicity and tissue tropism: Patho- 

 genic for sheep, causing abortion in the 

 pregnant female. When injected into sheep 

 subcutaneously, intravenously or intra- 

 dermally, a febrile response is produced. No 

 symptoms are produced when injected intra- 

 nasally or into the prepuce or the conjunc- 

 tiva. In the pregnant cow, produces abor- 

 tion with elementary bodies found in the 

 placenta. Produces no symptoms in calves 

 injected intranasally. No disease is pro- 

 duced in male guinea pigs. In the pregnant 

 female guinea pig after subcutaneous or 

 intraperitoneal injection, but not after in- 

 tracerebral injection, elementar}^ bodies are 

 found in the placenta. In mice and rats no 

 disease is produced by subcutaneous, intra- 

 peritoneal or intracerebral inoculation. In 

 mice, but not in rats, intranasal inoculation 

 produces pneumonitis. 



Habitat: The etiological agent of enzootic 

 abortion in ewes. Elementary bodies are 

 found in the placenta, fetal membranes and 

 uterine discharges. 



11. Miyagawanella bovis York and 

 Baker, 1951. (York and Baker, Jour. Exp. 

 Med., 93, 1951, 587; Rickettsia! ormis bovis 

 Zhdanov, Opredelitel Virusov Celovska i 

 Zivotmych, Izd. Akad. Med. Nauk, U.S. 

 S.R., Moskau, 1953, 187.) 



bo'vis. L. noun bos a cow; M.L. gen. noun 

 bovis of a cow. 



Coccoid cells. Stain red with Macchia- 

 vello's stain. 



Filterability: Passes through both Berke- 

 feldV andN filters. 



Cultivation: Grows particularly well in 

 the yolk sac of chicken embryos, less well 

 in the allantoic cavity and even less well on 

 the chorio-allantoic membrane. 



Immunology: Contains the group antigen 

 of the miyagawanellae, giving cross re- 

 actions in the neutralization test. A specific 

 neutralization can be obtained with chicken 

 sera, and the agent can be distinguished 

 from Miyagawanella felis. 



Toxic factor: Possesses a specific toxin as 

 in M. lymphogranulomatosis. 



Pathogenic for pigs, guinea pigs, mice, 

 cats, rabbits and chicken embryos. Pro- 

 duces an infection but no disease in calves. 

 Dogs are not susceptible. 



Tissue tropism: Appears in the feces of 

 calves after feeding. Mice are infected in- 

 tranasally only after passage and are not in- 

 fected by intraperitoneal or intracerebral 

 routes. Guinea pigs are infected intraperi- 

 toneally with the production of fever and 

 of peritonitis. Cats are infected either intra- 

 nasallj^or bj^ intracardiac injection with the 

 production of fever but are not infected per 

 OS. Rabbits are infected intraperitoneally 

 and pigs are infected intravenously with the 

 production of fever. 



Antibiotic- and chemo-therapy : Suscepti- 

 ble to aureomycin and penicillin but not to 

 sulfonamides. 



Habitat: Found in the feces of normal 

 calves, where no obvious disease is pro- 

 duced. 



12. Miyagawanella pecoris Rake, nom. 

 nov. (Agent of infectious encephalomyelitis 

 of cattle, McNutt, Vet. Med., 35, 1940, 228; 

 also see North Amer. Vet., 23, 1942, 242.) 



pe'co.ris. L. noun perns cattle (as a col- 

 lective herd); L. gen. noun pecoris of cattle. 



Elementary bodies are coccoid bodies 375 

 millimicrons in diameter when coated with 

 heavy metal. Staining reactions are similar 

 to those of Miyagawanella lymphogranulo- 

 matosis. 



Filterability: Passes through Berkefeld N 

 or V filters and fritted glass filters. 



Cultivation: Grows in the yolk sac of the 

 chicken embryo. 



Viable after storage at 24° C. for 227 days 

 and at -60° or -70° C. for 18 months. 



Immunology: Complement-fixing anti- 



