SECTION H 



1 . Aerobic 2 



Anaerobic; proceed to Section I p. 1029 



2. Cells 1.4 to 1.6 microns wide and 10 to 50 microns long, forming chains usually over 



100 micron.s in length; individual rods are characterized by constrictions which later 

 develop into cross walls followed by division; cells may branch, the branch appearing 

 at or near the site of constriction but not visibly connected with the main axis; 

 motile by means of peritrichous flagella; form small, bluish white, iridescent colonies 

 on peptone yeast extract agar, particularly with added acetate 



Lineola p. 832 

 Not as above 3 



3. Organisms isolated from cases of granuloma inguinale; grow only in the yolk sac of 



the developing chick embrj-o or in condensation water of a sloped medium prepared 

 by adding 50 per cent unheated embryonic j-olk to melted and cooled nutrient agar 



Calymmatobacterium -p. j^l8 

 Pleomorphic organisms parasitic on or within erythrocytes of man and other verte- 

 brates and cause infections of lower animals; stain by Giemsa's stain without visible 

 differentiation into nucleus and cytoplasm; grown with variable success in semi- 

 solid agar containing whole blood. See Manual keys for the family 



Bartonellaceae p. 968 



Organisms grow in glucose-blood-bouillon agar as Gram-negative rods 1.0 micron in 



length; occur in masses as coccoid and ellipsoidal cells 0.3 to 0.5 micron in diameter, 



extracellularly lining the intestinal epithelium of the sheep ked Melophagus ovinus 



Wolbachia p. 953 

 {W. melophagi) 

 Not as above 4 



4. Organisms isolated from nodules on the roots of leguminous plants and capable of 



producing nodules on the host plant Rhizobium p. 285 



Not as above 5 



5. Organisms capable of continued growth in a glucose or mannitol mineral salts medium 



devofd of nitrogen compounds 6 



Not as above 7 



6. Large, rod-shaped organisms; motile bj- means of peritrichous flagella 



Azotobacter p. 283 

 Small rods; motile by means of polar flagella .\zotomonas p. 198 



7. Endospores produced Bacillus p. 613 



Endospores not produced 8 



Note: Most species of the genus Bacillus are Gram -positive. 



8. Organisms grow well on yeast water medium containing up to 10 per cent alcohol, 



o.xidizing the latter to acetic acid; growth verj^ poor or absent on media containing 

 no carbohydrates Acetobacter p. 183 



Note: Erwinia carotovora is recorded to be able to oxidize 5 per cent ethyl 



alcohol. 

 Not as above 9 



9. Organisms grow poorly on peptone media in the absence of carbohydrates; good growth 



of white, raised, round colonies 1 mm in diameter on wort agar under anaerobic 

 conditions; only slight growth, if any, aerobicalh" in the presence of sugars; glucose 

 fermented with the production of carbon dioxide, lactic acid and up to 10 per cent 

 ethyl alcohol ; motile by means of a single polar flagellum .... Zymomonas p. 199 

 Organisms which will not grow on common laboratory media; grow in sucrose broth 

 containing less than 0.5 per cent NaCl and a very low concentration of meat ex- 



1015 



