COMPREHENSIVE KEY 1019 



Flagella polar; acid only in lactose; indole-negative; luminescent on a variety of 



media at pH 8.0 Photobaclerium p. 193 



Note: The polar flagellated plant pathogens Fseudomonas petasitis (colorless) 

 and Xanthomonas conjac (yellow pigmented), if it is lactose-positive, may ter- 

 minate here. 



Not as above: flagella peritrichous 47 



47. Alginate fermented with the production of acid and gas; hydrogen sulfide produced; 



methyl-red positive; Voges-Proskauer weak; citrate utilized; nitrite produced from 



nitrate Alginobacter p. 348 



Not as above 48 



48. Methyl-red positive; Voges-Proskauer negative 49 



Methyl-red negative; Voges-Proskauer positive 51 



Note: Erwinia nimipressuralis and E. dissolvens should be compared with 

 genera terminating between numbers 49 to 51 inclusive. 



49. Lactose fermented within 2 days 50 



Lactose fermentation delayed Paracolobactruin p. 3^6 



P. coZf/orme strains 28221, 551 1,661 1,261 1,31611, 111, 15411, 1811,33811 and 4361. 

 P. intermedium strains 14011, 12611 and 1421. 



Bethesda and Ballerup Groups, Rome, 1953, for gas-producing strains with slow 

 lactose fermentation. 



50. Acid and gas from lactose Escherichia p. 335 



{Escherichia, Rome, 1953, for motile strains) 



Acid only from lactose Paracolobactruin p. 346 



(P. intermedium strains 14011 and 12611) 



51. Lactose fermented in 2 days Aerobacter p. 341 



Lactose fermentation delayed Paracolobactruin p. 346 



(P. aerogenoides strains 721, 37711 and 37211) 



52. Organisms cause a glanders-like infection (melioidosis) in rats, guinea pigs, rabbits 



and man; colonies on agar small, circular, slightly raised, thick, opaque and cream- 

 colored with an irregular margin; litmus milk slowly coagulated; blood serum slowly 



liquefied; optimum temperature, 37°C Pseudomonas p. 89 



(P. pseudomallei) 



Organisms which produce granular conjunctivitis in monkeys and apes and which 

 are believed to be a cause of trachoma in man; optimum temperature, between 28° 

 and 30° C. with motility only at low temperatures; colonies on blood agar small, 

 circular, grayish and translucent, becoming sticky and mucoid 



Noguchia p. 4^1 



Not as above 53 



53. Organisms grow in 0.5 per cent peptone water containing a filter paper strip, weaken- 



ing the latter sufficiently to cause disintegration on slight agitation or reducing it to 

 a pulp; produce clearings around colonies on precipitated cellulose agar plates; 

 cells commonly arranged in angular fashion like corynebacteria 



Cellulonionas p. 601 

 (See also Pseudomonas arguta, P. tralucida and also P. ephemerocyanea if the latter 

 produces no pigment.) 

 Not as above 54 



54. Colonies yellow 55 



Colonies not yellow 57 



55. Flagella polar Xanthomonas p. 152 



(See also Pseudomonas perlurida, P. suhcreta, P. cepacia and also P. radiciperda 

 and Bacterium tardicrescens should the latter two be lactose fermenters.) 

 Flagella peritrichous 56 



