1022 COMPREHENSIVE KEY 



kauer negative; starch hydrolyzed ; pathogenic to the fish Salmonidae 



Aeromonas p. 189 

 Not as above : Voges-Proskauer positive 76 



76. Organisms encapsulated; cause respiratory and other infections in man 



Klebsiella p. 3U 



Not as above Paracolobactruni p. 346 



(P. aerogenoides strains 32011 and 32811) 



77. Obligate halophiles requiring 20 to 30 per cent salt for growth; pleomorphic; yellow 



colonies Halobacteriuin p. 207 



Not as above 78 



78. Organisms will not grow' on meat infusion agar without the addition of blood or ascitic 



fluid or X and V factors or other enrichments 79 



Not as above 80 



79. Pleomorphic coccobacillary organisms; grow well on blood agar producing colonies 



up to 4 mm in diameter on prolonged incubation; hydrogen sulfide produced from 

 cystine media; slight acid from glucose, fructose and mannose and possibly from 

 other sugars. Pathogenic, producing tularemia or tularemia-like infections in 



laboratory animals Pasteurella p. 395 



Organisms usually 0.2 to 0.5 micron wide and 0.5 to 2.0 microns long but frequently 

 produce long filaments; do not grow on nutrient agar or on MacConkey's lactose bile 

 salt agar; grow on nutrient agar with the addition of X factor or V factor or both or 

 with the addition of diphosphothiamine or adenosine triphosphate; colonies on suit- 

 able media rarely more than 1 mm in diameter after 2 days incubation; nitrites 

 produced from nitrates; various species have been reported as responsible for or 

 associated with viruses in: 



(i) Purulent meningitis and conjunctivitis in man (H. influenzae) 

 (ii) Sub-acute endocarditis {H. hemolyticus) 

 (iii) Acute and sub-acute conjunctivitis in man (H. aegyptius) 

 (iv) Soft chancre {H. ducreyi) 



(v) Vesicular eruptions in the genitals of cattle {H. citreus) 

 (vi) Ulcers of trout {H. piscium) 

 (vii) Commonly present in the respiratory tract of man (various species) and 



preputial secretions of dogs {H. haemoglobinophilus) 

 (viii) Pharyngitis {H. parahaemolyticus) 

 (ix) Non-pathogenic {H. parainfluenzae) 

 (x) Fowl coryza {H. gallinarum) 

 (xi) Swine influenza {H. suis) 



(xii) Respiratory tract of ferrets {H. putoriorum) Haemophilus p. ^06 



Note: This is the main entry for the genus Haemophilus. Owing to variable 

 biochemical characteristics, other entries occur in other parts of the key. 

 Fastidious organisms; require blood or ascitic fluid; highly pleomorphic; rod-shaped 

 or filamentous; may reach 100 to 150 microns in length; homogeneous in young cul- 

 tures but become granulated, beaded and swollen in old cultures and tend to frag- 

 ment into rods and cocci; large spherical swellings packed with granules common; 

 cholesterol globules appear among the growth; only rods and short filaments appear 

 in pathological material; non-branching; non-acid fast; colonies on ascitic agar 

 1 to 2.5 mm in 3 days; circular, low convex, colorless. L-phase variants are common. 

 Highly virulent for mice causing polyarthritis; causes rat-bite or Haverhill fever in 



man Streptobacillus p. 451 



{S. moniliformis) 



80. Slender rods; 1.0 to 3.0 microns long and often arranged in angular fashion; pleo- 



morphic; produce smooth, entire, butyrous, translucent, grayish yellow colonies 

 0.5 to 1.0 mm in diameter in 2 days at 37 °C. on meat infusion agar; may increase 



