40 THE ROYAL SOCIETY OF CANADA 



II, III and XV. 



V, XXII (and possibly lA). 



IB and XIX. 



X and XII. 



IV and XVIII. 



The others seem to differ so widely in some characteristics from 

 those of either of these groups and from one another as to leave little 

 doubt that they are separate species. They are not sufficiently well 

 characterized, nor have I had sufficient literature at my disposal, 

 to determine whether any of them are new. 



At least ten different species have therefore been isolated. Prob- 

 ably more were present in the water but were missed owing to the 

 specialized nature of the culture media used. For instance, No. (iv) 

 of the forms seen on direct examination of the surface sample was not 

 obtained in culture and no organism from the 100 fathom sample was 

 fully characterized though bacteria were undoubtedly present. 



The complex nature of the bacterial flora thus shown may be 

 due to the presence of fresh-water forms since the low density of the 

 water clearly indicated fresh water dilution. On the other hand the 

 fact that none of the organisms isolated would grow in fresh water 

 media seems to show an adaptation to saline conditions. It is hoped 

 at some future time to repeat the work with samples from such a 

 locality that this doubt cannot arise. 



In conclusion I wish to express my thanks to the late Dr. Wesbrook 

 and Dr. Mullin, the past and present professors of bacteriology at the 

 University of British Columbia, for their kindness in allowing me the 

 use of their laboratory in which this work was carried out. 



