Section V, 1919 [1351 Trans. R.S.C 



Further Work on the Bacteriology of Sivelled Canned Sardines 



By Wilfrid Sadler, M.Sc. and Irene Mounce, B.A., 



The University of British Columbia, Vancouver, 



and Eleanor Shanly, M.Sc, McGill University, Montreal 



Presented by Arthur Willey, F.R.S., D.Sc, F. R.S.C. 



(Read May Meeting, 1919.) 



The investigation into the "Bacteriology of Swelled Canned 

 Sardines" was begun by one of us in the summer of 1916, under the 

 auspices of the Biological Board of Canada. The work was done at 

 the Biological Station, St. Andrews, N.B., and in the laboratories 

 of Macdonald College (McGill). An Interim Report was published by 

 the Board in 1918 ;i and in March of the same year, a short paper was 

 presented to the Food and Drugs Section of the American Public 

 Health Association.^ 



The publications thus far have recorded the isolation and de- 

 tailed studies of eight strains of gas-producing bacteria. Of these, 

 two strains have been classified as B. vulgaris (Hauser, 1885), (Migula, 

 1900)^. The remaining six strains are typical and a-typical types of 

 the colon-aero genes group (Escherich)^. 



Experimental swellings of normal cans of sardines were accom- 

 pHshed by inoculation with three of the cultures respectively which 

 had been isolated from the original cans; and the "Postulates of Koch" 

 were satisfied. 



Present Paper 



The present paper is concerned with that phase of the investi- 

 gation which deals with the enquiry into the possible sources of the 

 causative bacteria. The investigation has been conducted partly on 

 the Atlantic coast from the Biological Station, St. Andrews a« the 

 centre; and partly in the laboratories at Macdonald College, and the 

 University of British Columbia, respectively. 



Methods 



The methods used in the collection of material for examination, 

 and in the subsequent cultural work, are, with some minor adapta- 

 tions, in conformity with those previously adopted and already 

 noted in the Interim Report.^ 



