[sadler-mounce-shanly] work ON BACTERIOLOGY 137 



Culture 22. — Litmus milk is bleached and digested; digestion is 

 almost complete in six days; acid to Methyl Red; negative to the 

 Voges-Proskauer reaction. Glucose, saccharose, maltose, xylose, 

 salicin and aesculin are fermented to acid and gas. Lactose, mannite 

 and dulcite are not fermented. 



Classification 



In spite of the differences exhibited by the three strains of this 

 group in certain of the reactions, we consider each to be a variety of 

 the type B. vulgaris (Hauser, 1885), (Migula, 1900) .» 



Group II 



Cultures 2, 9, 15, 17 and 18 are members of this group. They 

 have been isolated from the intestines of herrings, gills of herrings, 

 sea water used for pickle, and water from weirs respectively. 



Microscopically they are all short thick rods, gram negative, 

 and with the exception of culture 2, motile. Gelatine is not liquefied; 

 litmus milk is coagulated and bleached with formation of gas; indol 

 is not produced; lactose is fermented by each to acid and gas. In 

 certain other reactions some differences are to be noted. 



Culture 2. — Acid to Methyl Red; negative to the Voges-Proskauer 

 reaction. Glucose, lactose, maltose, mannite, xylose, salicin and aescu- 

 lin are fermented to acid and gas. Saccharose and dulcite are not 

 fermented. 



Culture 9. — Alkaline to Methyl Red; faintly positive to the Voges- 

 Proskauer reaction. Glucose, lactose, saccharose, maltose, mannite, 

 xylose and salicin are fermented to acid and gas. Dulcite and aesculin 

 are not fermented. 



Note: The fermentation of lactose is delayed, usually from two 

 to three days being required for the production of gas. 



Culture 15. — ^Alkaline to Methyl Red; positive to the Voges- 

 Proskauer reaction. Glucose, lactose, saccharose, maltose, mannite, 

 xylose, salicin and aesculin are fermented to acid and gas. Dulcite 

 is not fermented. 



Note: The fermentation of lactose is delayed, usually from two 

 to three days being required for the production of gas. 



Culture 17. — Acid to Methyl Red; negative to the Voges-Pros- 

 kauer reaction. Glucose, lactose, maltose, mannite, dulcite, xylose, 

 salicin and aesculin are fermented to acid and gas. Saccharose is 

 not fermented to gas. 



