64 THE ROYAL SOCIETY OF CANADA 



days medium slightly browned immediately beneath the 

 slope; growth echinulate as result of eliptical colonies grow- 

 ing back into medium from edge of growth. In three months 

 all traces of sliminess had disappeared, incubated at room 

 temperature. 



Loeffler's Blood Serum: 20 hrs. 37° C. growth abundant along 

 track of needle, compact with echinulate edges, glistening, 

 iridiscent, raised and slightly contoured, moist, creamy, 

 very slimy, no digestion. In 6 days growth more luxuriant, 

 slimy, no digestion; sliminess retained better than on any 

 other medium. 



Loeffler's Malachite Green Solution : 24 hrs. 37° C. weak coagulum 

 attached to sides and bottom of tube; bluish green liquid 

 exuded, much gas; slimy. In 40 hours sliminess gone, 

 liquid green. In 1 month no reduction, no digestion. 



Potato: 20 hrs. 37° C. growth moderate, wheat-straw colour, 

 glistening, smooth, slimy, edges slightly raised along track of 

 needle. In 48 hours raised, contoured, moist, luxuriant, 

 thick creamy. In 6 days sliminess maintained, growth 

 spins out into fine threads. 



Gelatine Stab: Room temperature, 24 hours growth filiform, 

 moderate, equally good surface and stab, no liquefaction. 

 In 48 hours growth abundant, below surface discrete to 

 beaded, gas, presumably from muscle sugar in beef extract, 

 no liquefaction. In 14 days growth in stab yellowish-brown, 

 beaded, medium slightly browned, no liquefaction. 



Nutrient Broth: 24 hours 37° C. clouding, slightly slimy, ring 

 at surface. In 48 hours heavy clouding of medium, slight 

 precipitate at bottom, sliminess in medium slight, but very 

 definite in ring at surface. In 14 days even clouding, heavy 

 viscid precipitate. 



Dunham's Solution: 24 hours 37° C. slimy, clouding. In 48 

 hours very heavy even clouding, "watered silk" appearance, 

 no pellicle, no precipitate, ring at surface slimy; sliminess 

 in medium gone. 



Dunham's Solution -\- Glucose -f di-potass. — hydrogen phosphate: 

 24 hours 37° C, heavy even clouding, slimy. In 48 hours 

 37°C. medium consistency of syrup. 



Giltay's Solution : 24 hours 37°C. slightly slimy, clouding. 



