[SADLER] A SLIME-PRODUCING ORGANISM 65 



Albumen Solution: (1% pure albumen in distilled water). 24 

 hours 37°C. growth, medium slimy; in 5 days sliminess still 

 persisting; no sliminess in 14 days. 



Casein Solution: Heavy clouding, slight sliminess, medium 

 syrupy. 



Bile Salt Broth: Heavy clouding, slightly slimy; slimy rim at 

 surface. In 5 days liquid not slimy, but surface rim dis- 

 tinctly slimy. 



Bile Salt Broth + Lactose: Heavy clouding of medium; con- 

 sistency syrupy, much gas, medium very slimy. 



Litmus Whey: Acid production, clouding, no flocculency, no 

 precipitate, gas, slimy, syrupy consistency. In 60 hours 

 liquid still slimy, surface rim very slimy. 



Milk: 20 hours 37°C. much froth, very slimy, no coagulation, 

 sliminess so pronounced that difiliculty in separating milk 

 from the needle. In 48 hours coagulated, w^hey expressed 

 1 cm. from surface, very frothy and much gas; smell "sweet 

 acid" typical of gas-forming group of organisms; exuded 

 whey slimy. In 6 days whey still slightly slimy, curd 

 riddled with gas holes. In 14 days no sliminess, no digestion. 

 In 6 wrecks curd shrunken, nO digestion. 



When large quantities of sterile milk are inoculated from a 

 young beef broth culture at the rate of 1 in 300, sliminess is 

 pronounced in 18 hours at 37°C., the milk roping to the 

 extent of two feet. Left subsequently at room temperature 

 in shallow vessels the pronounced sliminess has persisted for 

 several days, coagulation being delayed. After 6 days such 

 milk will "rope" for 2-3 feet. The organism produces pro- 

 nounced sliminess in whole milk, both in the cream layer 

 and in the separated sub-strata. 



Litmus Milk*: 20 hours 37°C. much froth, very slimy, lilaceus, 

 no coagulation. In 48 hours coagulation, pale lilaceus, curd 

 broken with gas; whey slimy. In 6 days bleached except at 

 surface. 



Aesculin Broth: In 12 hours black reaction. 



Aesculin agar: 36 hours, 37°C. colonies; surface colonies brown 

 by transmitted light, darker at centre, moist, shining, 

 raised to convex, compact, glistening, medium buff-colored. 

 Subsurface colonies black. Colonies very slimy. 



*Litmus made up in 7% solution in distilled water; sterilised and added to milk 

 immediately before inoculation at the rate of 1K%- 



Sec. IV, Sig.5 



