[HARRISON-SADLER] A BACTERIAL SOFT ROT OF TURNIPS 103 



30°C. were essentially as before, and as shown by the photo-micro- 

 graphs 3 and 5. On the other hand, the potato agar culture incu- 

 bated at 37°C. was entirely different, as the illustrations 1 and 6 depict. 



If the inoculations are reversed, some of the potato agar culture 

 grown at 37°C. being transferred to agar or potato agar and incubated 

 at 30°C,, the short rod form is in two transfers again observed. Each 

 microphotograph was made from a stained specimen prepared from 

 cultures 18 hours old. 



Gelatin Stab. Growth uniform and liquefaction commences in 

 24 hours; liquefaction saccate and complete in 7 days with a heavy 

 white flocculent growth at the bottom of the tube. 



Potato Gelatine. In six days liquefaction, which is of a saccate 

 nature, is complete; a more rapid and much more luxuriant growth 

 is obtained than in beef gelatin. The medium takes on a brownish 

 tint, and at the bottom of the tube there is a heavy flocculent pre- 

 cipitate. 



Gelatine Stroke. In 24 hours only slight growth can be observed, 

 with an aggregation of minute discrete colonies and a beginning of 

 liquefaction; in 8 days the gelatin is completely liquefied. 



Potato Gelatine Stroke. Rapid growth; liquefaction saccate, and 

 complete in 6 days. 



Nutrient Broth. In 24 hours slight clouding of the medium. In 

 48 hours growth and clouding increase with the formation of a ring 

 and the deposition of a flocculent sediment. 



Milk. In 24 hours no change is perceptible, the milk gradually 

 thickening, and in 6 days coagulation is complete and of a weak nature. 

 Expression of whey follows, but little or no peptonising, after three 

 months the appearance is the same. 



Litmus Milk. The action of the organism is similar to the above, 

 and when in six days coagulation is complete the milk is "lilacinus" 

 in colour. 



Nitrate Broth. No reduction of nitrate has been obtained when 

 tested after 4 days with the naphthylamine and sulphalinic acid so- 

 lutions. 



Dunharn's Solution. The organism does not produce indol, tested 

 after ten days' growth. 



Blood Serum at SO°C. Growth in this medium is good and rapid, 

 spreading over the surface and perceptible softening of the serum 

 being noticeable. This softening increases, the whole of the sloped 

 surface gradually becoming liquefied. 



Perm's Solution. Slight clouding and growth in 24 hours at 30°C. 



Cohn's Solution. No growth. 



