[SHUTT] NITROGEN-ENRICHMENT OF SOILS 119 
The alfalfa, as the clover, showed no benefit from the culture at 
the first cutting, indeed the “untreated” seed gave slightly the higher 
yield. One month later, however, when cut for the second time, the 
“soil inoculated” was somewhat the more vigorous crop, the yield 
exceeding that of either of the other members by about 12 per cent. 
When cut for the third time, an interval of seven weeks having elapsed, 
the growth from both the “seed” and “soil”. inoculated very consider- 
ably exceeded that from the untreated crop. A comparison of the total 
yields indicates a beneficial influence from both methods of inoculation, 
but most marked from that in which the soil is directly treated. 
In the field trials the culture was used only in treating the seed. 
The soil was a light sandy loam, fairly uniform as regards quality 
throughout the plots under investigation. The various cuttings were 
weighed as hay, the data being as follows: 
O.A.C. CULTURES—RED CLOVER AND ALFALFA—PLOT EXPERIMENT. 







~ Clover | Alfalfa 
| | 
From From | From From 
Untreated Inoculated Untreated Inoculated 
Seed Seed Seed Seed 
Lbs Oz Lbs Oz. | Lbs Oz Lbs Oz 
| 
| 
lSICUTEINES APE ONCE 15 15 20 12 29 Lele, 13 
2nd GC PA PR A AS 30 14 39 1 24 SN) ee 14 
3rd UT tg ER ed |e ee eRe Behn | Ae EURE 31 14 | 27 2 
Do le. Mio. 130 ESS NAN ES 



The difference in yields between the two plots of clover shows that 
this preparation has had a marked and beneficial influence: the increase 
attributable to the culture amounts to, approximately, 28 per cent. 
The heavier crop from alfalfa in all three cuttings was from the 
untreated seed. We can advance no adequate explanation that would 
account for the abnormally low yield from the inoculated seed, but 
that there was some depressing cause seems evident. 
Hiltner’s Nitragin: In the spring of 1909, trials were begun with 
Hiltner’s Nitragin as prepared by the Dr. Reiche Nitragin Co. 
Milwaukee, Wisconsin, U.S.A., cultures being used for red clover, 
alfalfa and peas. This is a fluid culture and the claim is made that not 
only is it in an easily applied form, but that “it can be kept for an 
